Growing and expanding primary, differentiated adult cells in culture is difficult and laborious because many differentiated cells can only be maintained in vitro for a limited period of time. However, for controlled experiments, it may be necessary to maintain a consistent population of cells.
Maintaining a population of adult stem cells (ASCs), allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired. This is possible with SACK cell strains and specified cell culture conditions.
SACK ASC cell properties – Long-term propagation in xanthosine (Xs)-supplemented medium
Differentiated progeny cell properties – Xs-free medium supplemented with 10% rat serum, confluent monolayer; or serum reduction and Xs-free medium supplemented with 10% bovine serum
*Additional similar, but less well characterized, independent clonal cell strains are available. For more information, contact us.
|Product Type:||Cell Line|
|Cell Type:||adult rat cholangiocytic stem cells|
|Source:||liver; derived from adult male Fischer 344 rats|
|Growth Conditions:||SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 400?M Xanthosine|
|Subculturing:||Passage 1:5 when cells reach approx. 80% confluence|
|Cryopreservation:||70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO|
Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.
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