Maintaining a population of adult stem cells (ASCs) allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired. This is possible with SACK cell strains and specified cell culture conditions.
SACK ASC Cell Properties
Differentiated progeny Cell Properties
*Additional similar, but less well characterized, independent clonal cell strains are available. For more information, contact us.
|Product Type:||Cell Line|
|Cell Type:||neonatal human liver stem cells|
|Source:||liver; 1 year old male donor cells|
|Growth Conditions:||SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 1.5mM Xanthosine|
|Subculturing:||Passage 1:5 when cells reach approx. 80% confluence|
|Cryopreservation:||70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO|
Example of stem cell quality control counting result for serial passage of the expanded neonatal human liver stem cell strain SACK-Xs 12(3) (300,000 initial total cell input). Note detection of symmetric cell division by stem cells between passages (vertical lines). Click here for more information regarding the QC process.
Express the epithelial stem cell biomarker LGR5 and the liver stem cell biomarker EpCAM.
Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.
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