Stable RAP Expression Plasmid

Plasmid containing the gene for Stable Receptor-Associated Protein (RAP).

Highlights:

  • Used to express RAP
  • Contains a stable RAP D3 domain that is resistant to both pH- and heat-induced denaturation

Stable Receptor-Associated Protein (RAP) is an antagonist of lipoprotein receptor-related protein 1 (LRP1). When RAP is administered IV post-stroke, it results in significant regaining of motor activity and a reduction in BBB leakage. RAP may also hold therapeutic promise in management of risk of bleeding in hemophiliac patients. The molecule binds to lipoprotein receptor-related protein 1 (LRP1) with high affinity and inhibits LRP1 function both in vitro and in vivo, suggesting that the novel RAP may be a useful tool in understanding lipoprotein metabolism, cell signaling, modulation of blood brain barrier integrity, and blood coagulation and fibrinolysis.

From the laboratory of Dudley K. Strickland, PhD, University of Maryland, Baltimore.

Catalog Number Product Size AVAILABILITY Price Qty
EMD008
Stable RAP Expression Plasmid
500ng In stock
Regular Price:$66.00
Specifications

Product Type: Plasmid
Gene/insert name: Stable Receptor Associated Protein (Stable RAP)
Organism: Human
Antibiotic Resistance: Ampicillin
Fusion Tag(s): GST
Format: Liquid
Grow in E. coli at 37 C: Yes
Cloning Site 5': BamH1
Cloning Site 3': EcoR1
Insert Size: 969 bp
Vector Backbone and Size: 4948 bp
High or low copy: High
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Dudley K. Strickland, PhD, University of Maryland, Baltimore.
References
  1. Prasad JM, Migliorini M, Galisteo R, Strickland DK. Generation of a Potent Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Antagonist by Engineering a Stable Form of the Receptor-associated Protein (RAP) D3 Domain. J Biol Chem. 2015 Jul 10;290(28):17262-8.

If you publish research with this product, please let us know so we can cite your paper.

Loading...