Complement C1s Proenzyme
Complement C1s proenzyme expressed in E. Coli.
C1s proenzyme is activated when the C1 complex binds to and is activated by antibodies bound to antigens (immune complexes) yielding C1r enzyme, the first protease that initiates the cascade. C1r enzyme in the C1 complex activates C1s proenzyme generating C1s enzyme. The activated C1s protease of the classical complement pathway propagates the initial activation of this pathway of the system by cleaving and thereby activating the C4 and C2 complement components. This facilitates the formation of the classical pathway C3 convertase (C4bC2a).
From the laboratory of Lakshmi C. Wijeyewickrema, PhD, La Trobe University.
Complement C1s proenzyme expressed in E. Coli.
C1s proenzyme is activated when the C1 complex binds to and is activated by antibodies bound to antigens (immune complexes) yielding C1r enzyme, the first protease that initiates the cascade. C1r enzyme in the C1 complex activates C1s proenzyme generating C1s enzyme. The activated C1s protease of the classical complement pathway propagates the initial activation of this pathway of the system by cleaving and thereby activating the C4 and C2 complement components. This facilitates the formation of the classical pathway C3 convertase (C4bC2a).
From the laboratory of Lakshmi C. Wijeyewickrema, PhD, La Trobe University.
| Product Type: | Protein |
| Name: | C1sCCP12SP Proenzyme |
| Accession ID: | P09871 |
| Source: | Escherichia coli |
| Molecular Weight: | 45156.23 (Predicted), around 50kDa on SDS-PAGE |
| Amino Acid Sequence: | K281-D688 |
| Purity: | >95%, by SDS-PAGE under reducing conditions and visualized by Coomassie Blue Stain |
| Buffer: | 20 mM Tris, 150mM Nacl, pH7.5 |
| Storage: | -80C |
| Shipped: | Dry Ice |
- Wijeyewickrema LC, Yongqing T, Tran TP, Thompson PE, Viljoen JE, Coetzer TH, Duncan RC, Kass I, Buckle AM, Pike RN. Molecular determinants of the substrate specificity of the complement-initiating protease, C1r. J Biol Chem. 2013 May 31;288(22):15571-80.
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