pBG218 Recombinant Sindbis Virus-GFP Expression Plasmid
Recombinant plasmid pBG218 expresses Sindbis virus (SINV) expressing GFP during viral replication for easy visualization.
Highlights:
- Plasmid can be transfected mammalian cells to produce infectious Sindbis virus
- Expresses Green Fluorescent Protein (GFP) for easy visualization of infection
The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. The gene for GFP was behind the 3' subgenomic promoter to allow expression of GFP protein during viral infection. Transfection of this construct into mammalian cells produces a self-replicating Sindbis virus genome that expresses GFP only when the virus genome is replicated in vitro or in vivo.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
Recombinant plasmid pBG218 expresses Sindbis virus (SINV) expressing GFP during viral replication for easy visualization.
Highlights:
- Plasmid can be transfected mammalian cells to produce infectious Sindbis virus
- Expresses Green Fluorescent Protein (GFP) for easy visualization of infection
The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. The gene for GFP was behind the 3' subgenomic promoter to allow expression of GFP protein during viral infection. Transfection of this construct into mammalian cells produces a self-replicating Sindbis virus genome that expresses GFP only when the virus genome is replicated in vitro or in vivo.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
| Product Type: | Plasmid |
| Gene/insert name: | Sindbis virus replicon |
| Organism: | Sindbis Virus Strain TE3'2J (AR339) |
| Antibiotic Resistance: | Amp |
| Fusion Tag(s): | eGFP |
| Grow in E. coli at 37 C: | DH5-alpha or XL10-Gold E. coli |
| Cloning Site 5': | MluI |
| Cloning Site 3': | XhoI |
| Insert Size: | 13,363 bp |
| Vector Backbone and Size: | pcDNA3.1 Zeo+ |
| High or low copy: | High |
| Storage: | Room Temperature (Dried), -20C (liquid) |
| Shipped: | Room Temperature |
- Geiss BJ, Shimonkevitz LH, Sackal CI, Olson KE. Recombination-ready Sindbis replicon expression vectors for transgene expression. Virol J. 2007 Oct 26;4:112.PubMed PMID: 17963504; PubMed Central PMCID: PMC2164957.
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