Recombinant plasmid pBG167 expresses infectious Sindbis virus (SINV) and allows for fast and easy expression of your gene of interest.
The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. A unique XbaI restriction site 3' subgenomic promoter to allow genes of interest to be ligated into the viral genome that will be expressed only when the virus genome is replicated. Transfection of this construct into mammalian cells produces an infectious self-replicating Sindbis virus genome that can be modified to express genes of interest in vitro or in vivo.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
|Gene/insert name:||Sindbis virus replicon|
|Organism:||Sindbis Virus Strain TE3'2J (AR339)|
|Grow in E. coli at 37 C:||DH5-alpha or XL10-Gold E-coli|
|Cloning Site 5':||MluI|
|Cloning Site 3':||XhoI|
|Insert Size:||12,169 bp|
|Vector Backbone and Size:||pcDNA3.1 Zeo+|
|High or low copy:||High|
|Storage:||Room Temperature (Dried), -20C (liquid)|
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