DLD-1 Colorectal Adenocarcinoma SIRT1 Knock Out Cell Line (DLD-1 SIRT1 KO)

Human Dukes-type C colorectal adenocarcinoma cell line, DLD-1 with a complete deletion of the Sirtuin 1 (SIRT1) gene.

SIRT1 is the most conserved mammalian NAD+-dependent protein deacetylase and an important cellular metabolic and stress sensor. Through deacetylation of transcription factors and co-factors critically involved in metabolic homeostasis, inflammatory responses, and stress resistance, SIRT1 directly couples cellular metabolic status (via NAD+) to transcriptional reprogramming in response to environmental changes.

From the laboratory of Xiaoling Li, PhD, National Institute of Environmental Health Sciences/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
DLD-1 Colorectal Adenocarcinoma SIRT1 Knock Out Cell Line (DLD-1 SIRT1 KO)
1 vial 4-6 weeks
Regular Price:$580.00
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Product Type: Cell Line
Name: DLD-1 SIRT1 KO
Cell Type: Human colorectal adenocarcinoma cell
Accession ID: Q96EB6
Organism: Homo sapiens, human
Source: Colon
Morphology: Epithelial
Biosafety Level: BSL 1
Subculturing: Adherent, 1:3 to 1:10 dilution
Growth Conditions: RPMI-1640 + 10% FBS
Cryopreservation: Complete growth medium supplemented with 10% DMSO
Storage: LN2
Shipped: Dry Ice

Human Dukes’ type C colorectal adenocarcinoma cell line, DLD-1, was transiently transfected with a Cas9WT mammalian expression vector expressing a gRNA targeting exon 5 (GGACAATTCCAGCCATCTCTCT) of human SIRT1 gene (Horizon Discovery, Cambridge, UK). Cell clones with complete deletion (KO) of human SIRT1 gene were identified by immunofluorescent staining, immunoblotting analysis and confirmed by genomic DNA PCR and sequencing. Cells were then cultured in RPMI1640 medium with 10% FBS.
From the laboratory of Xiaoling Li, PhD, National Institute of Environmental Health Sciences/NIH.
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  8. Vermeulen SJ, Chen TR, Speleman F, Nollet F, Van Roy FM, Mareel MM. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient? Cancer Genet Cytogenet. 1998 Nov;107(1):76-9. PubMed PMID: 9809040.
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