DLD-1 Colorectal Adenocarcinoma SIRT1 Knock Out Cell Line (DLD-1 SIRT1 KO)

Human Dukes-type C colorectal adenocarcinoma cell line, DLD-1 with a complete deletion of the Sirtuin 1 (SIRT1) gene.

SIRT1 is the most conserved mammalian NAD+-dependent protein deacetylase and an important cellular metabolic and stress sensor. Through deacetylation of transcription factors and co-factors critically involved in metabolic homeostasis, inflammatory responses, and stress resistance, SIRT1 directly couples cellular metabolic status (via NAD+) to transcriptional reprogramming in response to environmental changes.

From the laboratory of Xiaoling Li, PhD, National Institute of Environmental Health Sciences/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ENH130-FP
DLD-1 Colorectal Adenocarcinoma SIRT1 Knock Out Cell Line (DLD-1 SIRT1 KO)
1 vial 4-6 weeks
Regular Price:$499.00
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Specifications

Product Type: Cell Line
Name: DLD-1 SIRT1 KO
Cell Type: Human colorectal adenocarcinoma cell
Organism: Homo sapiens, human
Source: Colon
Morphology: Epithelial
Biosafety Level: BSL 1
Subculturing: Adherent, 1:3 to 1:10 dilution
Growth Conditions: RPMI-1640 + 10% FBS
Cryopreservation: Complete growth medium supplemented with 10% DMSO
Storage: LN2
Shipped: Dry Ice

Research
Human Dukes’ type C colorectal adenocarcinoma cell line, DLD-1, was transiently transfected with a Cas9WT mammalian expression vector expressing a gRNA targeting exon 5 (GGACAATTCCAGCCATCTCTCT) of human SIRT1 gene (Horizon Discovery, Cambridge, UK). Cell clones with complete deletion (KO) of human SIRT1 gene were identified by immunofluorescent staining, immunoblotting analysis and confirmed by genomic DNA PCR and sequencing. Cells were then cultured in RPMI1640 medium with 10% FBS.
Data
Provider
From the laboratory of Xiaoling Li, PhD, National Institute of Environmental Health Sciences/NIH.
References
  1. Ren NSX, Ji M, Tokar EJ, Busch EL, Xu X, Lewis D, Li X, Jin A, Zhang Y, Wu WKK, Huang W, Li L, Fargo DC, Keku TO, Sandler RS, Li X. Haploinsufficiency of SIRT1 Enhances Glutamine Metabolism and Promotes Cancer Development. Curr Biol. 2017 Feb 20;27(4):483-494.
  2. Chen TR, Hay RJ, Macy ML. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet Cytogenet. 1983 Dec;10(4):351-62. PubMed PMID: 6652615.
  3. Chen TR, Dorotinsky CS, McGuire LJ, Macy ML, Hay RJ. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet Cytogenet. 1995 Jun;81(2):103-8. PubMed PMID: 7621404.
  4. Trainer DL, Kline T, McCabe FL, Faucette LF, Feild J, Chaikin M, Anzano M, Rieman D, Hoffstein S, Li DJ, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int J Cancer. 1988 Feb 15;41(2):287-96. PubMed PMID: 3338874.
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  6. Rodrigues NR, Rowan A, Smith ME, Kerr IB, Bodmer WF, Gannon JV, Lane DP. p53 mutations in colorectal cancer. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7555-9. PubMed PMID: 1699228; PubMed Central PMCID: PMC54786.
  7. Keesee SK, Meneghini MD, Szaro RP, Wu YJ. Nuclear matrix proteins in human colon cancer. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1913-6. PubMed PMID: 8127905; PubMed Central PMCID: PMC43274.
  8. Vermeulen SJ, Chen TR, Speleman F, Nollet F, Van Roy FM, Mareel MM. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient? Cancer Genet Cytogenet. 1998 Nov;107(1):76-9. PubMed PMID: 9809040.
  9. Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

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