This cell line is a Lig3-/- derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were stably transfected with plasmid pMA2941, which encodes Cre recombinase. As a result of Cre-mediated excision, both Lig3 alleles in Cre4/2941#6 cells are inactive, which led to the loss of mtDNA.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblasts|
|Source:||Mouse E13.5 Embryo|
|Morphology:||Spindle-shaped at confluence|
|Biosafety Level:||BSL 1|
|Subculturing:||Split 1:4 every 3 days|
|Growth Conditions:||DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine, 1 mg/ml G418|
|Cryopreservation:||DMEM/10% FBS/ 1 mM Pyruvate/50 µg/ml uridine /10% DMSO|
If you publish research with this product, please let us know so we can cite your paper.