Cre4 Cell Line

This cell line was derived from mouse embryonic fibroblasts with loxP-targeted Lig3 alleles by immortalizing them with retrovirus rv3315, which encodes SV40 large T-antigen.

From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

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Cre4 Cell Line
1 vial In stock
Regular Price:$399.00
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Product Type: Cell Line
Cell Type: Embryonic fibroblasts
Organism: Mouse
Morphology: Spindle-shaped at confluence
Source: Mouse E13.5 Embryo
Biosafety Level: BSL 1
Growth Conditions: DMEM/10% FBS
Subculturing: Split 1:4 to 1:8 every 3 days
Cryopreservation: Media + 10% DMSO
Comments: Fast Growing
Storage: LN2
Shipped: Dry Ice

From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Cre-TM mice (JAX 004682, B6.Cg-Tg(CAG-cre/Esr1)5Amc/J) were intercrossed with Lig3LoxP mice (Nature 471(2011) 240-244) to provide tamoxifen-inducible cre expression driven from the actin promoter. Lig3Cre-TM mouse embryonic fibroblast (MEF) cell cultures were prepared from E13.5 embryonic mesenchyme. Cells were allowed to proliferate in T-25 tissue culture flasks at 37 °C in a humidified CO2-regulated (5%) incubator and were immortalized with a retroviral construct 3315, which encodes SV40 large T antigen (J. Biol. Chem. 288 (2013) 26594-26605) and selected depending upon their ability to form colonies. One clone was selected for its superior growth characteristics and designated Cre4.
  1. Shokolenko IN, Fayzulin RZ, Katyal S, McKinnon PJ, Wilson GL, Alexeyev MF. Mitochondrial DNA ligase is dispensable for the viability of cultured cells but essential for mtDNA maintenance. J Biol Chem. 2013 Sep 13;288(37):26594-605.

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