This cell line was derived from mouse embryonic fibroblasts with loxP-targeted Lig3 alleles by immortalizing them with retrovirus rv3315, which encodes SV40 large T-antigen.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblasts|
|Morphology:||Spindle-shaped at confluence|
|Source:||Mouse E13.5 Embryo|
|Biosafety Level:||BSL 1|
|Growth Conditions:||DMEM/10% FBS|
|Subculturing:||Split 1:4 to 1:8 every 3 days|
|Cryopreservation:||Media + 10% DMSO|
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