This cell line is a rho-0 derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were induced to lose their mtDNA by propagation in the medium containing 5 ug/ml EtBr. One of the resulting clones demonstrated an absence of mtDNA as determined by both PCR and Southern blotting and was designated Cre4 rho-0#11.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblasts|
|Source:||Mouse E13.5 Embryo|
|Morphology:||Spindle-shaped at confluence|
|Biosafety Level:||BSL 1|
|Subculturing:||Split 1:4 every 3 days|
|Growth Conditions:||DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine|
|Cryopreservation:||Media + 10% DMSO|
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