This cell line is a derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were engineered for Tet-On expression with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991), which encodes a Tet-On Advanced transactivator, EGFP, and blasticidin resistance, thus producing a clone designated 4B6.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblasts|
|Source:||Mouse E13.5 Embryo|
|Morphology:||Spindle-shaped at confluence|
|Biosafety Level:||BSL 1|
|Subculturing:||Split 1:4 to 1:8 every 3 days|
|Growth Conditions:||DMEM/10% FBS+10 µg/ml blasticidin|
|Cryopreservation:||Media + 10% DMSO|
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