HaloTag-RealThiol (HaloRT) Universal Organelle Specific Glutathione (GSH) Probe

The Halo-RealThiol (HaloRT) probe provides ratiometric fluorescent readout for quantification of glutathione in live mammalian cells.

Highlights:

  • HaloRT is cell permeable and can measure GSH levels in any organelle of interest
  • Conveniently compare organelle-specific GSH levels in live cells using confocal microscopy
  • Signals from blue and green channels allow for deduction of organelle specific GSH concentration, given appropriate calibration curve
  • Validated in the nucleus, cytosol, mitochondria, and endoplasmic reticulum (ER) in HeLa cells
  • This item is HaloRT ligand and must be used in cells expressing HaloTag protein

Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.

From the laboratory of Jin Wang, PhD, Baylor College of Medicine.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EBY004
HaloTag-RealThiol (HaloRT) Universal Organelle Specific Glutathione (GSH) Probe
50nmol In stock
Regular Price:$205.00
On Sale:
Specifications

Product Type: Small Molecule
Name: Halo-RealThiol (HaloRT) Ligand
Chemical Formula: C31H41ClN4O6
Molecular Weight: 601.14
Format: Red to orange solid (Lyophilized powder in tubes)
Purity: >97%, HPLC
Tested Applications: 1 µM for cell staining
Solubility: DMSO
Spectral Information: ex/em: HaloRT: 488/565; HaloRT-GSH: 405/488
Platform: Confocal microscopy/FACS
Compatible Cells: Mammalian cells e.g. HeLa, primary hepatocytes
Detection Method: Fluorescence
Comments: - Dilute DMSO stock with imaging buffer.
- A laser-based light source is preferred for best results.
- This item is HaloRT ligand and must be used in cells expressing HaloTag protein.
Storage: -80C
Shipped: Ambient Temperature

Provider
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
References
  1. Xiqian Jiang, Chengwei Zhang, Jianwei Chen, Sungwoo Choi, Ying Zhou, Mingkun Zhao, Xianzhou Song, Xi Chen, Mirjana Maletic-Savatic, Timothy Palzkill, David Moore, Meng C. Wang, and Jin Wang*, "Quantitative Real-Time Imaging of Glutathione with Subcellular Resolution", Antioxidants & Redox Signaling.
  2. Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article

If you publish research with this product, please let us know so we can cite your paper.

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