Cheilanthifoline synthase (CYP719A14) from Argemone mexicana produced in Sf9 insect cells is a P450 enzyme that catalyzes a methylenedioxy bridge on the molecule (S)-scoulerine to form (S)-cheilanthifoline, an essential step in the formation of benzo[c]phenanthridine and protoberberine alkaloids.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
|Molecular Weight:||55,898 Da|
|Buffer:||100 mM tricine pH 7.5/ 5 mM thioglycolic acid|
|Comments:||This enzyme requires the addition of NADPH for catalysis. Produced in Sf9 insect cells in conjunction with cytochrome P450 reductase from Eschscholzia californica and 2 mg/l hemin. Sf9 cells are permeabilized by the storage buffer and are able to be used directly in enzyme assays.|
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