Anti-SARS Non structural protein 8 (NSP8) [5A10] Antibody

This mouse IgG1 monoclonal antibody was raised against recombinant GST-NSP8 fusion protein and is specific for the non-structural protein 8 of SARS-Coronavirus (CoV).

Highlights:

  • Reacts with the non-structural protein 8 of SARS-CoV
  • Suitable for Western Blot and Immunofluorscence applications

Like other known coronaviruses, SARS-CoV is an enveloped, plus-strand RNA virus that features the largest RNA genomes currently known. SARS-CoV replicase polyproteins are processed by virus-encoded proteinases, and two proteinases have been identified: a 3C-like proteinase (3CLpro) and a papain-like proteinase (PLP). The processed end products of pp1a are designated nonstructural proteins (nsp) 1 to nsp11 and those of pp1ab are designated nsp1 to nsp16. Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein which is currently of undesignated function. The nsp8 protein has been indicated to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis.

From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.

Catalog Number Product Size AVAILABILITY Price Qty
ESG009
Anti-SARS Non structural protein 8 (NSP8) [5A10] Antibody
5mL supernatant 3-5 weeks
Regular Price:$250.00
Specifications

Product Type: Antibody
Antigen: Non-structural protein 8 of SARS-coronavirus
Molecular Weight: 22 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 5A10
Reactivity: SARS-CoV
Immunogen: Recombinant GST-NSP8 fusion protein
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IF
Storage: 80C
Shipped: Dry Ice

Data

Western Blot

Supernatant from 5A10 hybridoma used as primary antibody.

Provider
From the laboratory of Yee-Joo Tan, PhD, Agency for Science, Technology and Research (A*STAR).

References
  1. Meng B, Lui YW, Meng S, Cao C, Hu Y. Identification of Effective siRNA Blocking the Expression of SARS Viral Envelope E and RDRP Genes. Mol Biotechnol. 2006 Jun; 33(2):141 -8.
  2. Cheng A, Zhang W, Xie Y, Jiang W, Arnold E, Sarafianos SG, Ding J. Expression, purification, and haracterization of SARS coronavirus RNA polymerase. Virology. 2005 May; 335(2):165 -176.
  3. Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI. RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production. Proc Natl Acad Sci U S A 2005 Jan; 102(1):152-7.
  4. If you publish research with this product, please let us know so we can cite your paper.

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