This mouse IgG monoclonal antibody was generated against (DMPO), and recognizes DMPO-free thiol radicals.
Proteins and DNA can be easily isolated, immobilized and separated using standard techniques (gel electrophoresis, Western blotting, ELISA, cell fixation and permeabilization), before immunological detection of free radicals via the anti-DMPO monoclonal mouse antibody.
From the laboratory of Ronald P. Mason, PhD, National Institute of Environmental Health Sciences/NIH.
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|Antigen:||DMPO (5,5-Dimethyl-1-pyrroline N-oxide)|
|Accession ID:||CAS 3317-61-1|
|Molecular Weight:||N/A, molecular weight of DMPO = 113.16 g/mol|
|Immunogen:||DMPO-octanoic acid conjugated to albumin|
|Purification Method:||Protein G affinity chromatography|
|Method Used to Determine Concentration:||Absorbance at 280 nm (Abs of 1.35 = 1 mg/mL)|
|Buffer:||PBS (8 g/L NaCl, 0.2 g/L KCl, 1.15 g Na2HPO4.7H2O and 0.2 g/L KH2PO4, pH 7.4) with 0.02% azide and 10% glycerol.|
|Tested Applications:||WB 10 μg/mL, ELISA 10 μg/mL, ICC/IF 10 μg/mL|
|Comments:||Biological or in vitro samples must have DMPO present when free radicals were expected to be generated. The antibody is not indicated for immunoprecipitation.|
*Make up 1% casein/PBS by adding 10 gr casein to 100 ml 10 X PBS, bring up to 1 L with diH2O. Can stir overnight at 4 C to completely solubilize. Aliquot and freeze at -20 C.
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