This rabbit polyclonal antibody was generated against peptide and is specific for zebrafish vascular endothelial growth factor receptor kdr-like (Kdrl).
Kdrl is a receptor for VEGF or VEGFC with tyrosine-protein kinase activity. It's involved in angiogenesis specifically in VEGF-induced sprouting of new blood vessels and artery formation. Many VEGF receptors are required for development of different blood vessel types in the embryo.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
|Accession ID:||Q8AXB3, NP_571547|
|Molecular Weight:||147 kDa|
|Buffer:||Polyclonal serum with 0.02% sodium azide|
|Tested Applications:||Whole mount immunostaining (1:500)|
|Comments:||Recommend aliquotting into small working volumes after initial thaw and store these at -80°C|
Whole Mount Immunostaining
Whole mount immunostaining of a wild type zebrafish embryo at 24hpf using anti-zebrafish Kdrl antibody. Embryo was immunostained with primary anti-zebrafish Kdrl antibody at 1:5000, followed by detection using 1:5000 ZyMAXTM goat anti-rabbit IgG(H+L)-HRP (Life Technologies) and tyramide-Cy3 amplification. Strong fluorescence is observed in endothelial cells of the intersomitic vessels (indicated by arrows) and the dorsal aorta (DA).
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