This rabbit polyclonal antibody was generated against peptide and is specific for zebrafish vascular endothelial growth factor receptor 3 (Flt4).
Flt4 is known as a tyrosine-protein kinase that acts as a cell-surface receptor for vegf or vegfc. Flt4 is also involved in angiogenesis, specifically in VEGF-induced sprouting of new blood vessels, but not required for proper vasculogenesis or hematopoiesis.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
|Accession ID:||NP_571020, Q5MD89|
|Molecular Weight:||154 kDa|
|Buffer:||Polyclonal serum with 0.02% sodium azide|
|Tested Applications:||Immunoprecipitation, whole mount immunostaining (1:500)|
|Comments:||Recommend aliquotting into small working volumes after initial thaw and store these at -80°C|
Western Blot and Whole Mount Immunostaining
(A) Western blot of Flt4 immunoprecipitates from NIH3T3 cells transfected with a plasmid expressing full length zebrafish Flt4. Transfected cells were treated with conditioned medium (CM) from cells expressing zebrafish Vegfc, or from mock-transfected cells. Western blot was probed with antibodies against phosphotyrosine (pTyr) and zebrafish Flt4.
(B) Whole mount immunostaining using polyclonal antibody against zebrafish Flt4 on wild type and flt4null mutant embryos fixed at 30 hours post fertilization. Positive staining is denoted in the posterior cardinal vein (PCV) endothelial cells in wild type, but not flt4null mutant, embryos. Fluorescence signal was generated after primary immunostaining by using goat anti-rabbit IgG(H+L)-HRP followed by detection with tyramide-Cy3.
Adapted from: Villefranc JA et al., Development. 2013 Apr;140(7):1497-506.
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