This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish Fli1b protein (Fli1b).
Fli1b protein is a transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
|Molecular Weight:||51 kDa|
|Epitope:||N-terminal half of Fli1b|
|Buffer:||Polyclonal serum with 0.02% sodium azide|
|Tested Applications:||WB (1:5000), Whole mount immunostaining (1:500)|
|Comments:||Recommend aliquotting into small working volumes after initial thaw and store these at -80°C|
Upper panel: Whole mount immunohistochemistry, using 17-24 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Fl1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].
Lower panel: Whole mount immunohistochemistry of 48 hours post fertilization zebrafish embryos, visualizing head (left) and trunk (right) regions. Primary stain: anti-Fli1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen donkey anti-rabbit Alexa fluor 568 (Cat#:A10042); [1:1,000 Dilution].
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