This rat IgM monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, alpha-1,5-arabinan.
Isolated from a high throughput screen of antibodies generated subsequent to immunization with a pectic fraction. Recognizes a linear epitope in (1-5)-α-L-arabinans. Antibody recognition of arabinans increases with arabinofuranosidase action. This antibody binds to a specific subset of pectic arabinans, and to longer stretches of 1,5-linked arabinosyl residues that are likely to be more abundant in unbranched arabinans. The binding of LM13 is highly sensitive to arabinanase action. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
|Antigen:||Pectic polysaccharide, alpha-1,5-arabinan|
|Buffer:||Cell Culture Supernatant, 0.05% Sodium Azide|
|Tested Applications:||IF (1:10), ELISA (1:10)|
|Storage:||+4C (short term), -20C (long term)|
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