This rat IgG monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, beta-1,4-galactan.
The antibody was generated using a neoglycoprotein (galactotetraose-BSA). It recognizes a linear tetrasaccharide in (1-4)-Β-D-galactans. It has no cross-reactivity with (1-3)-Β-D-galactans or (1-6)-Β-D-galactans. It can recognize pectic polysaccharides in several species. In competitive inhibition ELISA, antibody binding to (1-4)-Β-D-galactan was inhibited (50%) by 58 μg/ml (1-4)-Β-D-galactotetraose and by 0.7 μg/ml lupin (1-4)-Β-D-galactan. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
|Antigen:||Pectic polysaccharide (beta 1,4-galactan)|
|Buffer:||Cell Culture Supernatant, 0.05% Sodium Azide|
|Tested Applications:||IF (1:10), ELISA (1:10)|
|Storage:||+4C (short term), -20C (long term)|
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