This rat IgM monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, homogalacturonan.
The antibody recognizes the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognize pectic polysaccharides in several species. The antibody requires methyl-esters for recognition of homogalacturonan and does not bind to un-esterified homogalacturonan. LM20 binding is more effectively lost by high pH treatments that remove HG methyl esters. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
|Antigen:||Pectic polysaccharide. Homogalacturonan|
|Epitope:||Methyl esterified homogalacturonan|
|Buffer:||Cell Culture Supernatant, 0.05% Sodium Azide|
|Tested Applications:||IF (1:10), ELISA (1:10)|
|Storage:||+4C (short term), -20C (long term)|
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