This rabbit polyclonal antibody was generated against a synthetic peptide and recognizes human, mouse, and rat phosphorylated (Ser113/Ser117) KH domain-containing, RNA-binding, signal transduction-associated protein 1 (Sam68/KHDRBS1).
Sam68 is a multifunctional nucleic acid binding protein involved in RNA splicing, RNA stability, transcriptional regulation signal transduction, and other is cells. Post-translation modifications on Sam68 are critical for its versatile functions. During T cell receptor engagement induced nulear factor-kappaB (NF-kB) activation, inhibitor of NF-kB kinase alpha (IKKα) phosphorylates Sam68 on serine 113 and serine 117 in the nucleus. Ser113 and Ser117 phosphorylated Sam68 complexes with NF-kB p65 subunit, facilitating NF-kB binding to selective promoters of genomic targets (Fu et al. Nat Commun. 2013 4:1909).
From the laboratory of Fengyi Wan, PhD, The Johns Hopkins University.
|Name:||phospho Sam68/KHDRBS1, Ser113/117 Antibody|
|Alternative Name(s):||KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, p21 Ras GTPase-activating protein-associated p62, p68|
|Molecular Weight:||68 kDa|
|Reactivity:||human, mouse, rat|
|Specificity:||phosphorylated Sam68 (Ser113 and Ser117)|
|Purification Method:||CNBr Sepharose Affinity Column|
|Buffer:||0.5M Tris-HCl, 0.25M NaCl, pH 8.0|
|Tested Applications:||WB (1:1000)|
(A) Whole cell lysates from mouse splenocytes stimulated with PMA plus ionomycin for as indicated periods were immunoprecipitated with isotype or Sam68 antibody. The immunoprecipitants were immunoblotted fro phosphorylated Sam68 and regular Sam68. (B) Cytosolic and nuclear fractions derived from Jurkat cells stimulated as indicated were directly immunoblotted (IB) for Sam68 phosphorylation with antibodies specific for S113/S117 phosphorylated Sam68 and general Sam68. Full-length Caspase 3 and PARP served as cytosolic and nuclear markers, respectively. Phosphorylated Sam68 is labelled by a triangle, and asterisks label nonspecific bands.
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