RealThiol (RT) Glutathione (GSH) Calibration Probe

RealThiol (RT) Detection Probe provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.


Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.

From the laboratory of Jin Wang, PhD, Baylor College of Medicine.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
RealThiol (RT) Glutathione (GSH) Calibration Probe
50nmol In stock
Regular Price:$218.00
On Sale:

Product Type: Small Molecule
Name: RealThiol (RT)
Chemical Formula: C20H17N3O7
Molecular Weight: 411.37
Format: Red to orange solid
Purity: >97%, HPLC
Solubility: DMSO
Spectral Information: ex/em: RealThiol: 488/565; RealThiol-GSH: 405/488
Platform: Confocal microscopy
Compatible Cells: For calibration only, NOT for cells
Detection Method: Fluorescence
Comments: - RT is for calibration only. For live cell applications, use RealThiol AM Ester (RT-AM) Glutathione Detection Probe
- Dilute DMSO stock with imaging buffer.
Storage: -80C
Shipped: Ambient Temperature

From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
  1. Jiang X, Chen J, Bajić A, Zhang C, Song X, Carroll SL, Cai ZL, Tang M, Xue M, Cheng N, Schaaf CP, Li F, MacKenzie KR, Ferreon ACM, Xia F, Wang MC, Maletić-Savatić M, Wang J. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.
  2. Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article

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