RealThiol (RT) Glutathione (GSH) Calibration Probe
RealThiol (RT) Detection Probe provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
Highlights:
- For use with: RealThiol AM Ester (RT-AM) Glutathione Detection Probe
- NOT cell permeable and used only in the solution phase to build calibration curves
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
RealThiol (RT) Detection Probe provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
Highlights:
- For use with: RealThiol AM Ester (RT-AM) Glutathione Detection Probe
- NOT cell permeable and used only in the solution phase to build calibration curves
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
Specifications
| Product Type: | Small Molecule |
| Name: | RealThiol (RT) |
| Chemical Formula: | C20H17N3O7 |
| Molecular Weight: | 411.37 |
| Format: | Red to orange solid |
| Purity: | >97%, HPLC |
| Solubility: | DMSO |
| Spectral Information: | ex/em: RealThiol: 488/565; RealThiol-GSH: 405/488 |
| Platform: | Confocal microscopy |
| Compatible Cells: | For calibration only, NOT for cells |
| Detection Method: | Fluorescence |
| Comments: |
- RT is for calibration only. For live cell applications, use RealThiol AM Ester (RT-AM) Glutathione Detection Probe - Dilute DMSO stock with imaging buffer. |
| Storage: | -80C |
| Shipped: | Ambient Temperature |
Documentation
Provider
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
References
- Jiang X, Chen J, Bajić A, Zhang C, Song X, Carroll SL, Cai ZL, Tang M, Xue M, Cheng N, Schaaf CP, Li F, MacKenzie KR, Ferreon ACM, Xia F, Wang MC, Maletić-Savatić M, Wang J. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.
- Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article
If you publish research with this product, please let us know so we can cite your paper.
