Human GM2 Ganglioside

This human ganglioside GM2 preparation was isolated from brain tissue of a patient who died of Sandhoff's disease.

Highlights:

  • Isolated from brain of deceased Sandhoff's disease patient
  • Highly pure - >95% (TLC)

Ganglioside GM2 is a minor component of cell membranes that has been shown to accumulate in Tay-Sachs and Sandhoff diseases. Gangliosides are glycosphingolipids, which are composed of an oligosaccharide linked to ceraminde, and additionally containing one or more silica acid residues.

From the laboratory of Don J. Mahuran, PhD, Hospital for Sick Children.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ESK002
Human GM2 Ganglioside
1mg Currently unavailable
Regular Price:$275.00
On Sale:
Specifications

Product Type: Protein
Name: GM2 ganglioside
CAS number: 19600-01-2
Accession ID: P17900
Source: human brain from patient who died of Sandhoff’s
Molecular Weight: 1383 Da
Amino Acid Sequence: beta-GalNac(1-4)[alpha-Neu5Ac(2-3)]beta-Gal(1-4)beta-Glc(1-1)Cer
Purity: >95% (TLC)
Storage: 4C dessicated (protect from moisture)
Shipped: Cold packs

Data

Purity of GM2 Ganglioside Preparation

Either 6 (left) or 18 (right) ug of GM2 ganglioside were resolved by silica gel thin layer chromatography using Chloroform:Methanol:Water (14:6:1) mobile phase. Positions of GM2 (major band) and GA2 (very minor band) were visualized using 50% Sulphuric Acid spray. Relative positions of GM2 and GA2 match those described in Novak et al, 1979. GM2 conversion to GA2 is accelerated by increased humidity, necessitating desiccated storage of the compound.

Provider
From the laboratory of Don J. Mahuran, PhD, Hospital for Sick Children.
References
  1. Novak A, Lowden JA, Gravel YL, Wolfe LS. Preparation of radiolabeled GM2 and GA2 gangliosides. J Lipid Res. 1979 Jul;20(5):678-81.

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