This human ganglioside GM2 preparation was isolated from brain tissue of a patient who died of Sandhoff's disease.
Ganglioside GM2 is a minor component of cell membranes that has been shown to accumulate in Tay-Sachs and Sandhoff diseases. Gangliosides are glycosphingolipids, which are composed of an oligosaccharide linked to ceraminde, and additionally containing one or more silica acid residues.
From the laboratory of Don J. Mahuran, PhD, Hospital for Sick Children.
|Source:||human brain from patient who died of Sandhoff’s|
|Molecular Weight:||1383 Da|
|Amino Acid Sequence:||beta-GalNac(1-4)[alpha-Neu5Ac(2-3)]beta-Gal(1-4)beta-Glc(1-1)Cer|
|Storage:||4C dessicated (protect from moisture)|
Purity of GM2 Ganglioside Preparation
Either 6 (left) or 18 (right) ug of GM2 ganglioside were resolved by silica gel thin layer chromatography using Chloroform:Methanol:Water (14:6:1) mobile phase. Positions of GM2 (major band) and GA2 (very minor band) were visualized using 50% Sulphuric Acid spray. Relative positions of GM2 and GA2 match those described in Novak et al, 1979. GM2 conversion to GA2 is accelerated by increased humidity, necessitating desiccated storage of the compound.
If you publish research with this product, please let us know so we can cite your paper.