7-Dehydrocholesterol (d7 isotope)

Isotopically labeled sterol intermediate in the cholesterol biosynthesis pathway. Reacts with the dienophile, 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) to give products that have unique MS fragmentation patterns. This assay increases the sensitivity and shortens the analysis time.

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

Catalog Number Product Size AVAILABILITY Price Qty
EVU123
7-Dehydrocholesterol (d7 isotope)
1mg In stock
Regular Price:$300.00
Specifications

Product Type: Small Molecule
Name: 7-Dehydrocholesterol-25,26,26,26,27,27,27-d7
Chemical Formula: C27H37D7O
Source: synthetic
Molecular Weight: 391.69
Format: white powder
Purity: >95%, NMR
Isotopic Purity: 94%
Solubility: CHCl3, THF, benzene
Mass Shift: m/z 7
Comments: susceptible to oxidation
Storage: -80C
Shipped: Dry ice

Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
  1. The Effect of Small Molecules on Sterol Homeostasis: Measuring 7-Dehydrocholesterol in Dhcr7-Deficient Neuro2a Cells and Human Fibroblasts. Korade, Z.; Kim, H.-Y. H.; Tallman, K. A.; Liu, W.; Koczok, K.; Balogh, I.; Xu, L.; Mirnics, K.; Porter, N. A. J. Medicinal Chemistry 2016, 59, 1102.
  2. A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome. Liu, W.; Xu, L.; Lamberson, C.; Haas, D.; Korade, Z.; Porter, N. A. J. Lipid Research 2014, 55, 329.
  3. Korade Z, Genaro-Mattos TC, Tallman KA, Liu W, Garbett KA, Koczok K, Balogh I, Mirnics K, Porter NA. Vulnerability of DHCR7+/- mutation carriers to aripiprazole and trazodone exposure. J Lipid Res. 2017 Sep 28. pii: jlr.M079475. View Article
  4. Genaro-Mattos TC, Tallman KA, Allen LB, Anderson A, Mirnics K, Korade Z, Porter NA. Dichlorophenyl piperazines, including a recently-approved atypical antipsychotic, are potent inhibitors of DHCR7, the last enzyme in cholesterol biosynthesis. Toxicol Appl Pharmacol. 2018 Jun 15;349:21-28. View Article
  5. Wages PA, Kim HH, Korade Z, Porter NA. Identification and Characterization of Prescription Drugs that Change Levels of the Cholesterol Precursors 7 Dehydrocholesterol and Desmosterol. View Article
  6. Genaro-Mattos TC, Allen LB, Anderson A, Tallman KA, Porter NA, Korade Z, Mirnics K. Maternal aripiprazole exposure interacts with 7-dehydrocholesterol reductase mutations and alters embryonic neurodevelopment. Mol Psychiatry. 2019 Apr;24(4):491-500. View Article

If you publish research with this product, please let us know so we can cite your paper.

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