This rabbit polyclonal antibody was generated against thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) and recognizes human, mouse, and rat TXNDC8.
Thioredoxin domain-containing protein 8 (TXNDC8, SPTRX-3) may be required for post-translational modification of proteins required for acrosomal biogenesis. TXNDC8 may act by reducing disulfide bonds within the sperm. The protein is testis-specific, and is only expressed during spermiogenesis, prominently in the Golgi apparatus of pachytene spermatocytes and round and elongated spermatids, with a transient localization in the developing acrosome of round spermatids. TXNDC8 accumulates in superfluous cytoplasm of defective human spermatozoa.
From the laboratory of Peter Sutovsky, PhD, University of Missouri-Columbia.
|Alternative Name(s):||Spermatid-specific thioredoxin-3|
|Antigen:||TXNDC8, SPTRX-3, Thioredoxin-6|
|Molecular Weight:||20 kDa|
|Reactivity:||Human, Mouse, Rat|
|Immunogen:||Mixture of peptides from sequences encoded within exon I [(NH2-) MVQIIKDTNEFKTFC (-COOH)] and exon V [(Ac-) VTLFSRIKRIIC (-COOH)] of murine TXNDC8|
|Purification Method:||Immunogen affinity purification|
|Buffer:||affinity purified rabbit serum (pH 7.2, 0.05% sodium azide)|
|Tested Applications:||WB 1:2000, ELISA 1:200-1:2000, Flow cytometry 1:100, IHC 1:100, IF 1:100|
|Comments:||Positive control: Human spermatozoa (IF), testicular tissue homogenate (WB, ELISA), or tissue sections (IHC)|
|Storage:||Upon delivery, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles|
Representative experimental data for TXNDC8 (SPTRX-3) Antibody.
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