Anti-CYE-1 [17C8] Antibody, (supernatant)
![Anti-CYE-1 [17C8] Antibody, (supernatant)](https://www.kerafast.com/MediaStorage/Product/Images/Large/1394_2001202001264613930.jpg "Anti-CYE-1 [17C8] Antibody, (supernatant)")
This mouse monoclonal antibody was generated against full-length recombinant cyclin E (CYE-1) protein and is specific for Caenorhabditis elegans CYE-1 full-length recombinant protein (prepared in bacteria).
Highlights:
- Recognizes C. elegans CYE-1 full-length recombinant protein (prepared in bacteria)
- Suitable for Immunofluorescence and Western Blot applications
Cyclin E is a member of the cyclin family of proteins, which regulates the cell cycle through its activation of cyclin-dependent kinases. Specifically, cyclin E binds and activates the S phase Cdk2. The cyclin E–Cdk2 complex promotes the G1 to S phase cell cycle transition. Overexpression of cyclin E has been implicated in carcinomas among the gastrointestinal tract, including colon or stomach cancer as well as a marker for breast cancers.
From the laboratory of Edward T. Kipreos, PhD, University of Georgia.
This mouse monoclonal antibody was generated against full-length recombinant cyclin E (CYE-1) protein and is specific for Caenorhabditis elegans CYE-1 full-length recombinant protein (prepared in bacteria).
Highlights:
- Recognizes C. elegans CYE-1 full-length recombinant protein (prepared in bacteria)
- Suitable for Immunofluorescence and Western Blot applications
Cyclin E is a member of the cyclin family of proteins, which regulates the cell cycle through its activation of cyclin-dependent kinases. Specifically, cyclin E binds and activates the S phase Cdk2. The cyclin E–Cdk2 complex promotes the G1 to S phase cell cycle transition. Overexpression of cyclin E has been implicated in carcinomas among the gastrointestinal tract, including colon or stomach cancer as well as a marker for breast cancers.
From the laboratory of Edward T. Kipreos, PhD, University of Georgia.
| Product Type: | Antibody |
| Accession ID: | NM_001025857.2 |
| Antigen: | C. elegans CYE-1 full-length recombinant protein (prepared in bacteria) |
| Molecular Weight: | 60.6 kDa |
| Clonality: | Monoclonal |
| Clone Name: | 17C8 |
| Reactivity: | C. elegans |
| Immunogen: | Full-length recombinant CYE-1 protein |
| Species Immunized: | Mouse |
| Purification Method: | Not purified, available as antibody supernatant |
| Buffer: | Cell culture supernatant |
| Tested Applications: | Immunofluorscence (1:100-300) and Western Blot |
| Storage: | -80C |
| Shipped: | Cold Packs |
- Brodigan, T.M., Liu, J., Park, M., Kipreos, E.T., and Krause, M. 2003. Cyclin E expression during development in C. elegans. Developmental Biology 254: 102-115.
- Kim, Y., and Kipreos, E.T. 2007. The C. elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex. Molecular and Cellular Biology 27: 1394-1406.
- The, I., Ruijtenberg, S., Bouchet, B. P., Cristobal, A., Prinsen, M. B., van Mourik, T., Koreth, J., Xu, H., Heck, A. J., Akhmanova, A., Cuppen, E., Boxem, M., Munoz, J., & van den Heuvel, S. (2015). Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells. Nat Commun, 6, 5906.
- Fox, P. M., Vought, V. E., Hanazawa, M., Lee, M. H., Maine, E. M., & Schedl, T. (2011). Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline. Development, 138 , 2223-34.
- Chiang, M., Cinquin, A., Paz, A., Meeds, E., Price, C. A., Welling, M., & Cinquin, O. (2015). Control of Caenorhabditis elegans germ-line stem-cell cycling speed meets requirements of design to minimize mutation accumulation. BMC Biol, 13, 51.
- Korzelius, J., The, I., Ruijtenberg, S., Prinsen, M. B., Portegijs, V., Middelkoop, T. C., Groot, M. J. K., Holstege, F. C., Boxem, M., & van den Heuvel, S. (2011). Caenorhabditis elegans cyclin D/CDK4 and cyclin E/CDK2 induce distinct cell cycle re-entry programs in differentiated muscle cells. PLoS Genet, 7, e1002362.
- Friend, K., Campbell, Z. T., Cooke, A., Kroll-Conner, P., Wickens, M. P., & Kimble, J. (2012). A conserved PUF-Ago-eEF1A complex attenuates translation elongation. Nat Struct Mol Biol, 19, 176-83.
- Peel, N., Dougherty, M., Goeres, J., Liu, Y., & O'Connell, K. F. (2012). The C. elegans F-box proteins LIN-23 and SEL-10 antagonize centrosome duplication by regulating ZYG-1 levels. J Cell Sci, 125, 3535-44.
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