Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) that are pluripotent and have the appearance of both neuronal and glial morphologies in culture.
suprachiasmatic nucleus or nuclei (SCN) is a tiny region of the brain in the hypothalamus, situated directly above the optic chiasm. It is responsible for controlling circadian rhythms. Pathologies and environmental disturbances in the regulation of circadian rhythms have been linked to variety of human health disorders including obesity and diabetes, increased cancer risk, cardiovascular disease, sleep-wake cycle disturbances, and depression.
From the laboratory of David J. Earnest, PhD, Texas A&M University.
|Product Type:||Cell Line|
|Cell Type:||Mixed Astrocytes & Neurons|
|Source:||Brain: Suprachiasmatic Nucleus|
|Growth Conditions:||MEM (Gibco #10370) Minimal Essential Media + non-essential amino acids, 10% FBS, 0.9 ml 45% glucose per 100 ml MEM, 1 ml L-glutamine (Gibco #25030-081, 200 mM) per 100 ml MEM, Optional: 0.5 ml gentamycin (50 ug/ml, Gibco #15710-064) or 1ml pen-strep (100ug,U/ml,)|
|Subculturing:||See attached protocol|
|Cryopreservation:||Growth media w/ 7.5% DMSO|
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