Rat Suprachiasmatic Nucleus Cell Line (SCN 2.2)

Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) that are pluripotent and have the appearance of both neuronal and glial morphologies in culture.

Highlights:

  • Capable to differentiate into different phenotypes
  • Expresses the neuronal markers (NSE, PGP, and MAP-2), but absent of glial markers
  • Valuable tool for studies relating to the regulation of the central mammalian pacemaker function, and the development of the suprachiasmatic nucleus (SCN) cell types

suprachiasmatic nucleus or nuclei (SCN) is a tiny region of the brain in the hypothalamus, situated directly above the optic chiasm. It is responsible for controlling circadian rhythms. Pathologies and environmental disturbances in the regulation of circadian rhythms have been linked to variety of human health disorders including obesity and diabetes, increased cancer risk, cardiovascular disease, sleep-wake cycle disturbances, and depression.

From the laboratory of David J. Earnest, PhD, Texas A&M University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EUT500
Rat Suprachiasmatic Nucleus Cell Line (SCN 2.2)
1 vial In stock
Regular Price:$699.00
On Sale:
Specifications

Product Type: Cell Line
Cell Type: Mixed Astrocytes & Neurons
Accession ID: CVCL_D050
Source: Brain: Suprachiasmatic Nucleus
Organism: Rat
Morphology: Polymorphic
Biosafety Level: BSL1
Growth Conditions: MEM (Gibco #10370) Minimal Essential Media + non-essential amino acids, 10% FBS, 0.9 ml 45% glucose per 100 ml MEM, 1 ml L-glutamine (Gibco #25030-081, 200 mM) per 100 ml MEM, Optional: 0.5 ml gentamycin (50 ug/ml, Gibco #15710-064) or 1ml pen-strep (100ug,U/ml,)
Subculturing: See attached protocol
Cryopreservation: Growth media w/ 7.5% DMSO
Storage: Liquid nitrogen
Shipped: Dry Ice

Provider
From the laboratory of David J. Earnest, PhD, Texas A&M University.
References
  1. Earnest DJ, Liang FQ, DiGiorgio S, Gallagher M, Harvey B, Earnest B, Seigel G. Establishment and characterization of adenoviral E1A immortalized cell lines derived from the rat suprachiasmatic nucleus. J Neurobiol. 1999 Apr;39(1):1-13.

If you publish research with this product, please let us know so we can cite your paper.

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