This mouse monoclonal antibody was generated against a mammalian expressed cohesin-hAMIGO2 fusion protein and recognizes human Amphoterin-induced protein 2 (AMIGO2).
Amphoterin-induced protein 2 (AMIGO2), also known as Alivin1, contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. AMIGO2 may contribute to signal transduction through its intracellular domain. AMIGO2 may be required for tumorigenesis of a subset of gastric adenocarcinomas.
From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.
|Antigen:||cohesin-Amigo2 Ecto domain|
|Molecular Weight:||60,374 Da (fusion protein) Ecto domain of Amigo2 (40-397)|
|Immunogen:||Mammalian expressed C-terminal fusion protein. N-terminal HisTag-cohesin-hAmigo2 (residues 40-397)|
|Species Immunized:||mouse BalbC (myeloma fusion with SP2-0/Ag14)|
|Purification Method:||MabSelect (ProA Affinity)|
|Tested Applications:||ELISA, FACS, IHC|
|Storage:||Long term -80C; Short term 4C; avoid freeze thaws|
Immunohistochemistry of paraffin-embedded skin utilizing Anti-hAMIGO2 30G9.1G2. (A) Representative images of IHC performed on paraffin-embeded skin. (B) Isotype control shows clear contrast to actual staining pattern with AMIGO2 antibody.
(A) Anti-hAMIGO2 30G9.1G2 on CHO cells stably expressing full length AMIGO2. (B) Anti-hAMIGO2 30G9.1G2 on CHO cells. Purified Mabs stained at 2 ug/ml; 500000 cells/pt, Secondary staining with 1:250 goat anti-mouse IgG PE (BD Biosciences); PBS 2% BSA staining buffer used.
If you publish research with this product, please let us know so we can cite your paper.