Anti-AMIGO2 [30G9.1G2] Antibody

This mouse monoclonal antibody was generated against a mammalian expressed cohesin-hAMIGO2 fusion protein and recognizes human Amphoterin-induced protein 2 (AMIGO2).

Highlights:

  • Specific for human AMIGO2
  • Suitable for ELISA, FACS, and Immunohistochemistry applications

Amphoterin-induced protein 2 (AMIGO2), also known as Alivin1, contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. AMIGO2 may contribute to signal transduction through its intracellular domain. AMIGO2 may be required for tumorigenesis of a subset of gastric adenocarcinomas.

From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.

Catalog Number Product Size AVAILABILITY Price Qty
EBS001
Anti-AMIGO2 [30G9.1G2] Antibody
100ug In stock
Regular Price:$306.00
Specifications

Product Type: Antibody
Antigen: cohesin-Amigo2 Ecto domain
Accession ID: NP_862830
Molecular Weight: 60,374 Da (fusion protein) Ecto domain of Amigo2 (40-397)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: AB60_71.30G9.1G2
Reactivity: Human
Immunogen: Mammalian expressed C-terminal fusion protein. N-terminal HisTag-cohesin-hAmigo2 (residues 40-397)
Species Immunized: mouse BalbC (myeloma fusion with SP2-0/Ag14)
Purification Method: MabSelect (ProA Affinity)
Buffer: PBS (MgCa++)
Tested Applications: ELISA, FACS, IHC
Storage: Long term -80C; Short term 4C; avoid freeze thaws
Shipped: Cold packs

Data

Immunohistochemistry

Immunohistochemistry of paraffin-embedded skin utilizing Anti-hAMIGO2 30G9.1G2. (A) Representative images of IHC performed on paraffin-embeded skin. (B) Isotype control shows clear contrast to actual staining pattern with AMIGO2 antibody.

FACS

(A) Anti-hAMIGO2 30G9.1G2 on CHO cells stably expressing full length AMIGO2. (B) Anti-hAMIGO2 30G9.1G2 on CHO cells. Purified Mabs stained at 2 ug/ml; 500000 cells/pt, Secondary staining with 1:250 goat anti-mouse IgG PE (BD Biosciences); PBS 2% BSA staining buffer used.

Provider
From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.
References

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