Anti-AMIGO2 [30G9.1G2] Antibody

This mouse monoclonal antibody was generated against a mammalian expressed cohesin-hAMIGO2 fusion protein and recognizes human Amphoterin-induced protein 2 (AMIGO2).

Highlights:

  • Specific for human AMIGO2
  • Suitable for ELISA, FACS, and Immunohistochemistry applications

Amphoterin-induced protein 2 (AMIGO2), also known as Alivin1, contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. AMIGO2 may contribute to signal transduction through its intracellular domain. AMIGO2 may be required for tumorigenesis of a subset of gastric adenocarcinomas.

From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EBS001
Anti-AMIGO2 [30G9.1G2] Antibody
100ug In stock
Regular Price:$306.00
On Sale:
Specifications

Product Type: Antibody
Antigen: cohesin-Amigo2 Ecto domain
Accession ID: NP_862830
Molecular Weight: 60,374 Da (fusion protein) Ecto domain of Amigo2 (40-397)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: AB60_71.30G9.1G2
Reactivity: Human
Immunogen: Mammalian expressed C-terminal fusion protein. N-terminal HisTag-cohesin-hAmigo2 (residues 40-397)
Species Immunized: mouse BalbC (myeloma fusion with SP2-0/Ag14)
Purification Method: MabSelect (ProA Affinity)
Buffer: PBS (MgCa++)
Tested Applications: ELISA, FACS, IHC
Storage: Long term -80C; Short term 4C; avoid freeze thaws
Shipped: Cold packs

Data

Immunohistochemistry

Immunohistochemistry of paraffin-embedded skin utilizing Anti-hAMIGO2 30G9.1G2. (A) Representative images of IHC performed on paraffin-embeded skin. (B) Isotype control shows clear contrast to actual staining pattern with AMIGO2 antibody.

FACS

(A) Anti-hAMIGO2 30G9.1G2 on CHO cells stably expressing full length AMIGO2. (B) Anti-hAMIGO2 30G9.1G2 on CHO cells. Purified Mabs stained at 2 ug/ml; 500000 cells/pt, Secondary staining with 1:250 goat anti-mouse IgG PE (BD Biosciences); PBS 2% BSA staining buffer used.

Provider
From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.
References
  1. Lin LL, Kost ER, Lin CL, Valente P, Wang CM, Kolonin MG, Daquinag AC, Tan X, Lucio N, Hung CN, Wang CP, Kirma NB, Huang TH. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer. Cell Rep. 2020 Oct 13;33(2):108253.  View Article

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