Recombinant HuD/ELAVL4 Neoantigen

Recombinant HuD/ELAVL4 neoantigen expressed in E. coli and purified via affinity chromatography.

Highlights:

  • Paraneoplastic Antigen for detection of anti-Hu antibodies/AUUUA element binding Protein

ELAV-like protein 4 (ELAVL4, HuD) plays a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). HuD binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA. HuD was originaly discovered as the paraneoplastic antigen expressed in neuroendocrine tumors. Recombinant HuD was first used to assay the presence of anti-Hu antibodies and thereby diagnose the presence of small cell lung cancer and subacute sensory neuronopathy. In normal cells , it functions to regulate gene expression and the recombinant protein has been used to identify regualtory elements in mRNA.

Catalog Number Product Size AVAILABILITY Price Qty
FCG004
Recombinant HuD/ELAVL4 Neoantigen
10ug In stock
Regular Price:$380.00
Specifications

Product Type: Protein
Name: Recombinant HuD Protein
Accession ID: P26378
Source: Recombinant from E. coli
Format: Liquid
Buffer: PBS, pH 7.3
Tested Applications: Detection of anti-Hu paraneoplastic antibodies via WB or ELISA assay. Identification of regulatory elements in mRNA.
Storage: -20C
Shipped: Ambient temp. Upon delivery, aliquot and store at 4C (short term) or -20C (long term).

Data

EMSA Assay and SDS-PAGE Analysis

(A) HuD selectively binds to AU element in c-fos mRNA.(B) SDS Gel analysis of recombinant HuD.

References

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