pGL3-Gpr177_3 plasmid is a vector encoding the mammalian homologue of Drosophilia Wntless and can be used to transfect cells and use luciferase to monitor Wntless promoter activity.
Gpr177 is the mammalian homologue of Drosophila Wntless (Wls; aka Evi, Srt) is required for Wnt secretion in Wnt-producing cells. Wntless binds to lipid modified Wnts, and is mainly localized in the Golgi and trafficking vesicles to modulates intracellular sorting of Wnt proteins. It has been demonstrated that genetic inactivation of Wntless in mice causes similar defects to the loss of canonical Wnt as well as non-canonical Wnt signaling. However, the loss of Wntless does not affect Wnt expression but Wnt secretion in the signal-producing cells. Wntless is also associated with the retromer complex to recycle Wnt from the plasma membrane. The expression pattern of Wntless provides critical information for Wnt-producing cells and source of Wnt in development and disease. The pBSSK-Gpr177 plasmid can generate RNA sense and anti-sense probe to detect Wntless/Gpr177 expression in various tissues, e.g. primitive streak, hippocampus, midbrain, choroid plexus, thalamus, spinal cord, tooth, tongue, palate, hair follicle, inner ear, olfactory epithelium, eye, muscle, nerve, salivary gland, kidney, lung, thymus, esophagus, duodenum, by in situ hybridization.
From the laboratory of Wei Hsu, PhD, University of Rochester.
|Gene/insert name:||Wntless/Gpr177 promoter -563~+55|
|Fusion Tag(s):||Luciferase reporter|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||Xhol|
|Cloning Site 3':||HindIII|
|Insert Size:||618 bp|
|Vector Backbone and Size:||4818|
|High or low copy:||High|
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