pcDNA-3FHP Hepatitis B Virus (HBV) Polymerase Protein Expression Plasmid
This pcDNA-3FHP plasmid contains the coding sequence for the hepatitis B virus (HBV) polymerase protein (HP) with an N-terminal 3x-Flag tag, downstream of the CMV promoter in the pcDNA3 backbone.
Highlights:
- Can be used to express the HBV polymerase protein in mammalian cells
Hepatitis B virus is a species of the genus Orthohepadnavirus, which is likewise a part of the Hepadnaviridae family of viruses. This virus causes the disease hepatitis B that attacks the liver and can be prevented by getting vaccinated.
From the laboratory of Jianming Hu, MD, PhD, Penn State College of Medicine.
This pcDNA-3FHP plasmid contains the coding sequence for the hepatitis B virus (HBV) polymerase protein (HP) with an N-terminal 3x-Flag tag, downstream of the CMV promoter in the pcDNA3 backbone.
Highlights:
- Can be used to express the HBV polymerase protein in mammalian cells
Hepatitis B virus is a species of the genus Orthohepadnavirus, which is likewise a part of the Hepadnaviridae family of viruses. This virus causes the disease hepatitis B that attacks the liver and can be prevented by getting vaccinated.
From the laboratory of Jianming Hu, MD, PhD, Penn State College of Medicine.
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | HBV polymerase |
| Antibiotic Resistance: | Amp |
| Fusion Tag(s): | 3xFlag (N-terminal) |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Neomycin |
| Cloning Site 5': | EcoRV |
| Cloning Site 3': | EcoRV |
| Insert Size: | ca. 2500 bp |
| Vector Backbone and Size: | pcDNA3 (ca. 5400 bp) |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Liquid as Ambient temperature |
Provider
From the laboratory of Jianming Hu, MD, PhD, Penn State College of Medicine.
References
- Jones SA, Boregowda R, Spratt TE, Hu J. In vitro epsilon RNA-dependent protein priming activity of human hepatitis B virus polymerase. J Virol. 2012 May;86(9):5134-50.
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