Mouse Embryonic Fibroblast Bcl-xL Knock Out Cell Line (MEF Bcl-xL-KO)

Mouse embryonic fibroblast cell line generated from a mouse embryo lacking B-cell lymphoma-extra-large (Bcl-xL) expression.

Also available: Mouse Embryonic Fibroblast Bcl-xL Expressing Cell Line (MEF wild-type)

B-cell lymphoma 2 (Bcl-2) proteins are major regulators of cellular responses to intrinsic and extrinsic apoptotic stimuli. Among them, overexpression of the antiapoptotic protein Bcl-xL modulates intracellular Ca2+ homeostasis and organelle-specific apoptotic signaling pathways. Bcl-xL is a transmembrane molecule in the mitochondria and acts as a pro-survival protein by preventing the release of mitochondrial contents such as cytochrome c, which would lead to caspase inactivation. Relative amounts of pro- and anti-survival Bcl-2 family of proteins define whether the cell will undergo cell death. If more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. However, if Bcl-2 members Bax and Bak become activated, and Bcl-xL is sequestered away by gatekeeper BH3-only factors (e.g., Bim), causing a pore to form, cytochrome c is released leading to initiation of caspase cascade leading to apoptotic events.

From the laboratory of Chi Li, PhD, University of Louisville.

Catalog Number Product Size AVAILABILITY Price Qty
EUL012
Mouse Embryonic Fibroblast Bcl-xL Knock Out Cell Line (MEF Bcl-xL-KO)
1 vial In stock
Regular Price:$799.00
Specifications

Product Type: Cell Line
Name: MEF (Bcl-xL-KO)
Cell Type: Fibroblast
Organism: Murine
Accession ID: CVCL_HC60
Morphology: Cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate
Source: Embryos
Biosafety Level: BSL2
Subculturing: 1/10 split
Growth Conditions: DMEM + 10% fetal bovine serum
Cryopreservation: fetal bovine serum + 10% DMSO
Storage: Liquid nitrogen
Shipped: Dry ice

Provider
From the laboratory of Chi Li, PhD, University of Louisville.
References
  1. Eno CO, Eckenrode EF, Olberding KE, Zhao G, White C, Li C. Distinct roles of mitochondria- and ER-localized Bcl-xL in apoptosis resistance and Ca2+ homeostasis. Mol Biol Cell. 2012 Jul;23(13):2605-18.
  2. Smith ML, Tahir SK. Quantification of BCL-2 Family Members by Flow Cytometry. Methods Mol Biol. 2019;1877:163-172. View Article

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