Mouse embryonic fibroblast cell line generated from a mouse embryo lacking B-cell lymphoma-extra-large (Bcl-xL) expression.
B-cell lymphoma 2 (Bcl-2) proteins are major regulators of cellular responses to intrinsic and extrinsic apoptotic stimuli. Among them, overexpression of the antiapoptotic protein Bcl-xL modulates intracellular Ca2+ homeostasis and organelle-specific apoptotic signaling pathways. Bcl-xL is a transmembrane molecule in the mitochondria and acts as a pro-survival protein by preventing the release of mitochondrial contents such as cytochrome c, which would lead to caspase inactivation. Relative amounts of pro- and anti-survival Bcl-2 family of proteins define whether the cell will undergo cell death. If more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. However, if Bcl-2 members Bax and Bak become activated, and Bcl-xL is sequestered away by gatekeeper BH3-only factors (e.g., Bim), causing a pore to form, cytochrome c is released leading to initiation of caspase cascade leading to apoptotic events.
From the laboratory of Chi Li, PhD, University of Louisville.
|Product Type:||Cell Line|
|Morphology:||Cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate|
|Growth Conditions:||DMEM + 10% fetal bovine serum|
|Cryopreservation:||fetal bovine serum + 10% DMSO|
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