This mouse IgGk monoclonal antibody was generated against recombinant protein and recognizes human, mouse and rat protein kinase C delta type (PRKCD, PKC δ).
The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
From the laboratory of Anumantha G. Kanthasamy, PhD, Iowa State University.
|Accession ID:||Q05655, NP_006245|
|Antigen:||Protein kinase C delta type (PRKCD, PKC δ)|
|Molecular Weight:||77.5 kDa|
|Species Immunized:||Prkcd knock out mice|
|Buffer:||Cell Culture Supernatant|
|Tested Applications:||Western Blot (1: 500), ELISA (1:1000), IHC (1:50)|
Western Blot Analysis
Data shows PKC delta highly localized in granular cell layer of cerebellum. Experimental Conditions: Antigen retrieval by microwave method. Blocking buffer 3% goat serum 1% triton-X100 in PBS. O/N PRIMARY 1:200 FOR NeuN 1:1000 o/n with secondary. O/N SECONDARY 1:2000
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