Anti-PKC Delta [10F11B] Antibody (supernatant)

This mouse IgGk monoclonal antibody was generated against recombinant protein and recognizes human, mouse and rat protein kinase C delta type (PRKCD, PKC δ).

Highlights:

  • Reacts with human, mouse and rat protein kinase C delta type (PRKCD, PKC δ)
  • Suitable for Western Blot, ELISA and Immunhistochemistry applications

The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

From the laboratory of Anumantha G. Kanthasamy, PhD, Iowa State University.

Catalog Number Product Size AVAILABILITY Price Qty
EIS003
Anti-PKC Delta [10F11B] Antibody (supernatant)
5mL (supernatant) In stock
Regular Price:$256.00
Specifications

Product Type: Antibody
Accession ID: Q05655, NP_006245
Antigen: Protein kinase C delta type (PRKCD, PKC δ)
Molecular Weight: 77.5 kDa
Isotype: IgGk
Clonality: Monoclonal
Clone Name: 10F11B
Immunogen: Recombinant protein
Species Immunized: Prkcd knock out mice
Buffer: Cell Culture Supernatant
Tested Applications: Western Blot (1: 500), ELISA (1:1000), IHC (1:50)
Storage: -80
Shipped: Dry ice

Data

Western Blot Analysis

Immunohistochemistry Analysis

Data shows PKC delta highly localized in granular cell layer of cerebellum. Experimental Conditions: Antigen retrieval by microwave method. Blocking buffer 3% goat serum 1% triton-X100 in PBS. O/N PRIMARY – 1:200 FOR NeuN 1:1000 o/n with secondary. O/N SECONDARY – 1:2000

Provider
From the laboratory of Anumantha G. Kanthasamy, PhD, Iowa State University.
References

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