E. coli strain with expressing capsid 10A with a C-terminal biotinylation tag for phage display studies.
Bacteriophage T7 is a bacteriophage capable of infecting susceptible bacterial cell (mostly E. coli). Bacteriophage T7 is a DNA virus that has a lytic life cycle. The T7 bacteriophage has several properties that make it an ideal phage for experimentation.
From the laboratory of Wei Li, PhD, University of Miami.
|Cell Type:||E. coli|
|Genotype:||F? , ompT, hsdSB (rB ? , mB ? ), dcm, gal, 10A-Biotin|
|Growth Conditions:||Lysogeny broth (LB) or Terrific broth (TB) medium|
|Transformation:||Culture BLT7Bio bacteria until the OD600 reaches to 0.5; add IPTG (1 mM) to bacteria and continue shaking for 30 min. BLT7Bio can be stored at 4C for up to 2 days; add T7 phage at appropriate titer to the bacteria and shake at 37C until lysis.|
|Induction:||IPTG for 30 min at 37C with shaking|
|Storage:||Uninduced BLT7Bio should be stored at -80C for long-term storage; IPTG-induced BLT7Bio should be stored at 4C for 2 days.|
T7 phage amplified in BLT7Bio bacteria will have all surface capsid 10A (~400 copies/phage) displaying biotin. This will not affect foreign proteins displayed on C-terminus of capsid 10B.Use horseradish peroxidase-conjugated streptavidin to detect bound phage amplified in BLT7Bio bacteria.
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