4PD in vivo transfection kit is a nanocarrier for the selective drug delivery to and transfection of myeloid-derived suppressor cells (MDSCs) and macrophages (Mφ) in vivo.
Myeloid-Derived Suppressor Cells (MDSCs) and tumor-associated macrophages (Mφ) are emerging as the most important pro-tumoral populations present in tumor host. These cells play a key role in promoting tumor progression and metastasis as well as in inhibiting spontaneous or therapeutically induced anti-tumor immunity. The 4PD nanoparticle is a G5 PAMAM dendrimer functionalized with a peptides recognizing receptors on MDCSs and macrophages. The formulation has been optimized with the use of amphipathic molecules that increase specificity and reduce the immunogenicity of the nanoparticle. Nucleic acids (e.g., DNA, RNA, shRNA), small molecules, drugs, etc. are loaded onto the dendrimer and the amphipathic molecule is added to reduce immunogenicity. 4PD has been shown effective in specifically targeting and delivering nucleic acids to MDSCs and serves as a useful tool for detection or the modulation of gene expression.
From the laboratory of Paolo Serafini, PhD, University of Miami.
|Name:||4PD in vivo MDSC/Mφ Transfection Kit|
Component 1: 4PD
Component 2: Enhancing solution
Component 3: 10X buffer
Component 4: Endotoxin/RNAse-free water
Transfection of primary human MDSCs and macrophages
Transfection reagent to study complex MDSC pathways in vivo
Regulation of gene expression in MDSCs in vivo and in vitro
Therapeutic delivery platform to reverse tumor-mediated immunosuppression
Following the fate of transfected cells by flow cytometry
|Number of Transfections:||Sufficient material for 30 injections of 10ug of RNA in mice|
|Molecule Delivered:||Nucleic acids (e.g., DNA, RNA, shRNA), small molecules, drugs|
|Injection Route:||Systemic delivery (Tail vein, intravenous, intraperitoneal injection), Local injection|
|Particle Size:||60-100nm (fully loaded)|
|Storage:||-20C >1 year|
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