This mouse IgG monoclonal antibody was raised against isolated rat hepatocyte gap junctions and recognizes human, mouse, and rat Gap junction beta-1 protein (Connexin-32).
Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
From the laboratory of David L. Paul, PhD, Harvard University.
|Antigen:||Connexin-32, Gap junction beta-1 protein, Cx32, Gjb1|
|Molecular Weight:||32,007 Da|
|Reactivity:||Mouse, rat, human|
|Immunogen:||isolated rat hepatocyte gap junctions|
|Epitope:||On cytoplasmic surface, likely between residues 95-125|
|Purification Method:||Serum separation from whole blood|
|Tested Applications:||IHC (acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested. Works with lightly formaldehyde-fixed tissue). WB (Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates).|
|Storage:||-80C long term, 4C short term|
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