Anti-Connexin-32 [M12.13] Antibody

This mouse IgG monoclonal antibody was raised against isolated rat hepatocyte gap junctions and recognizes human, mouse, and rat Gap junction beta-1 protein (Connexin-32).

Highlights:

  • Recognizes human, mouse and rat Connexin-32 on the cytoplasmic surface (residues 95-125)
  • Suitable for Western Blot and Immunohistochemistry

Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

From the laboratory of David L. Paul, PhD, Harvard University.

Catalog Number Product Size AVAILABILITY Price Qty
EF0013
Anti-Connexin-32 [M12.13] Antibody
100ug 4-6 weeks
Regular Price:$306.00
Specifications

Product Type: Antibody
Antigen: Connexin-32, Gap junction beta-1 protein, Cx32, Gjb1
Accession ID: NM_017251.2
Molecular Weight: 32,007 Da
Isotype: IgG
Clonality: Monoclonal
Clone Name: M12.13
Reactivity: Mouse, rat, human
Immunogen: isolated rat hepatocyte gap junctions
Species Immunized: Mouse
Epitope: On cytoplasmic surface, likely between residues 95-125
Purification Method: Serum separation from whole blood
Buffer: Whole serum
Tested Applications: IHC (acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested. Works with lightly formaldehyde-fixed tissue). WB (Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates).
Storage: -80C long term, 4C short term
Shipped: Dry ice

Provider
From the laboratory of David L. Paul, PhD, Harvard University.
References
  1. Goodenough DA, Paul DL, Jesaitis L. 1988. Topological distribution of two Connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. J.Cell Biol. 107:1817-1824.
  2. Hamzei-Sichani F, Davidson KG, Yasumura T, Janssen WG, Wearne SL, Hof PR, Traub RD, Gutiérrez R, Ottersen OP, Rash JE. Mixed Electrical-Chemical Synapses in Adult Rat Hippocampus are Primarily Glutamatergic and Coupled by Connexin-36. Front Neuroanat. 2012 May 15;6:13.
  3. Rela L, Bordey A, Greer CA. Olfactory ensheathing cell membrane properties are shaped by connectivity. Glia. 2010 Apr 15;58(6):665-78. doi: 10.1002/glia.20953.
  4. Fowler SL, McLean AC, Bennett SA. Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system. Cell Commun Adhes. 2009 Dec;16(5-6):117-30.
  5. Rash JE, Olson CO, Pouliot WA, Davidson KG, Yasumura T, Furman CS, Royer S, Kamasawa N, Nagy JI, Dudek FE. Connexin36 vs. connexin32, "miniature" neuronal gap junctions, and limited electrotonic coupling in rodent suprachiasmatic nucleus. Neuroscience. 2007 Oct 26;149(2):350-71. Epub 2007 Jul 21.
  6. Rash JE, Olson CO, Davidson KG, Yasumura T, Kamasawa N, Nagy JI. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007 Jul 29;147(4):938-56. Epub 2007 Jul 2.
  7. Fischer R, Reinehr R, Lu TP, Schönicke A, Warskulat U, Dienes HP, Häussinger D. Intercellular communication via gap junctions in activated rat hepatic stellate cells. Gastroenterology. 2005 Feb;128(2):433-48.
  8. Rash JE, Pereda A, Kamasawa N, Furman CS, Yasumura T, Davidson KG, Dudek FE, Olson C, Li X, Nagy JI. High-resolution proteomic mapping in the vertebrate central nervous system: close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1). J Neurocytol. 2004 Jan;33(1):131-51.
  9. Alvarez-Maubecin V, Garcia-Hernandez F, Williams JT, Van Bockstaele EJ. Functional coupling between neurons and glia. J Neurosci. 2000 Jun 1;20(11):4091-8.
  10. Day WE, Bowen JA, Barhoumi R, Bazer FW, Burghardt RC. Endometrial connexin expression in the mare and pig: evidence for the suppression of cell-cell communication in uterine luminal epithelium. Anat Rec. 1998 Jul;251(3):277-85.

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