RS-14 - Large Stokes-Shift NIR Dye (499-599 Ex/582-760 Em)-Acid
RS-14 is a near-infrared fluorescent dye with an excitation of 499-599nm and emission of 582-760nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
RS-14 is a near-infrared fluorescent dye with an excitation of 499-599nm and emission of 582-760nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
| Product Type: | Small Molecule |
| Name: | 2-(1-hydroxy- 10-oxo-10H-benzo[c] xanthen-7-yl)-benzoic acid, methyl ester |
| CAS number: | 1380077-21-3 |
| Chemical Formula: | C25H16O5 |
| Extinction Coefficient: | 13,318 in MeOH,12,168 in pH 6, 8613 in pH 12 |
| Molecular Weight: | 396.39 |
| Tested Applications: | pH sensitive red or NIR emission |
| Ex/Em Maxima: | Emission at 582 in MeOH, 600 in pH 6, 760 in pH 12 |
| Abs Max (nm): | 501,536 in MeOH, 499, 530 in pH 6. 599 in pH 12 |
| Detection Method: | Fluorescence |
| Storage: | -20C; protect from light |
| Shipped: | Cold packs |
- Sibrian-Vazquez M, Escobedo JO, Lowry M, Strongin RM. Progress toward red and near-infrared (NIR) emitting saccharide sensors. Pure Appl Chem. 2012 Nov 1;84(11):2443-2456.
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