Recombinant protein purified from E. coli, consisting of GST fused to the Cdc42 (p21) binding domain (CRIB) of the human p21 activated kinase 1 protein (PAK).
This non-radioactive pulldown assay uses the p21 binding domain (PBD) of the Rac/Cdc42, p21-activated protein kinase (PAK1). This domain is expressed as an N-terminal GST-fusion protein coupled to glutathione beads and can be used to isolate the active, GTP-bound form of Rac/Cdc42 from whole cell lysates. After precipitation, Rac/Cdc42 can be detected by immunoblotting with the appropriate antibodies.
From the laboratory of Jorrit M. Enserink, PhD, University of Oslo.
|Molecular Weight:||34 kDa GST fusion protein|
|Amino Acid Sequence:||RSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSA|
|Buffer:||30% glycerol, PBS pH7.4, 2 mM EDTA, 10 mM DTT, protease inhibitors|
Recommended amount of beads per assayed sample: 2-2.5 µl (higher amounts are unnecessary and may even negatively affect experimental outcome).
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