This mouse IgG monoclonal antibody was generated against epsilon Toxoid (A single amino acid mutant of whole toxin) and recognizes clostridium perfringens epsilon toxin.
The epsilon toxin is one of 12 protein toxins produced by Clostridium perfringens, a Gram positive, anaerobic spore-forming rod. There are five strains of C. perfringens, designated A through E. Each strain produces a unique spectrum oftoxins. The epsilon toxin is made by types B and D. This toxin is a pore-forming protein; it causes potassium and fluid leakage from cells. In addition to the epsilon toxin, C. perfringens type D strains produce alpha toxin and type B strains produce alpha and beta toxins. C. perfringens type B causes severe enteritis in young calves, foals, lambs and piglets. Type D causes enterotoxemia in sheep and goats and, on rare occasions, in cattle. All five strains can infect wounds in any species.
From a laboratory at Iowa State University.
|Antigen:||C. perfringensEpsilon Toxin|
|Molecular Weight:||35 kDa|
|Immunogen:||Epsilon Toxoid (A single amino acid mutant of whole toxin)|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||Western Blot (0.5ug/mL)|
Purified 4D6 anti-ETX antibody is used in this western blotting at a final concentration of 0.5ug/ml. They used Anti-Mouse-IgG-HRP as secondary antibody at 1:3000 dilution. Western is developed using TMB, liquid substrate system for membranes. Lanes 1: 625pg of His-Epsilon Toxoid, Lane 2: 1.25ng of His-Epsilon Toxoid, Lane 3: 2.5ng of His-Epsilon Toxoid, Lane 4: 5ng of His-Epsilon Toxoid, Lane 5 : 10ngs of His-Epsilon Toxoid, Lane 6: SeeBluePlus2 Protein-Marker, Lane 7: Semi-pure, ~ 5ngs ETX proto-toxin, Lane 8: Trypsin activated, 5ngs of ETX proto-toxin, Lane 9 : Trypsin activated, 2.5ngs of ETX proto-toxin.
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