This mouse monoclonal antibody was generated against a biotin-keyhole limpet hemocyanin conjugate and recognizes free and haptenic biotin and biocytin.
Biotin is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
From the laboratory of Alec H. Sehon, PhD, University of Manitoba.
|Antigen:||Biotin ( tested for ability to inhibit biotin-dependent enzymes)|
|Immunogen:||biotin-keyhole limpet hemocyanin conjugate|
|Species Immunized:||female BALB/c mice|
|Purification Method:||Protein G|
|Buffer:||PBS, pH 7.4|
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