pA-B(D1080A)-CD RecBCD Expression Plasmid
This plasmid expresses a set of plasma borne genes that encode a nuclease-deficient mutant form of the bacterial enzyme RecBCD, which unwinds double-stranded DNA very rapidly and with high processivity.
Highlights:
- Encodes an Asp-1080-to-Ala mutation in the RecB subunit - Abolishes the nuclease activity, but retains rapid and processive helicase activity.
RecBCD (EC 3.1.11.5, Exonuclease V, Escherichia coli exonuclease V, E. coli exonuclease V, gene recBC endoenzyme, RecBC deoxyribonuclease, gene recBC DNase, gene recBCD enzymes) is an enzyme of the E. coli bacterium that initiates recombinational repair from potentially lethal double strand breaks in DNA which may result from ionizing radiation, replication errors, endonucleases, oxidative damage, and a host of other factors. The RecBCD enzyme is both a helicase that unwinds, or separates the strands of DNA, and a nuclease that makes single-stranded nicks in DNA.
From the laboratory of Douglas A. Julin, PhD, University of Maryland, College Park.
This plasmid expresses a set of plasma borne genes that encode a nuclease-deficient mutant form of the bacterial enzyme RecBCD, which unwinds double-stranded DNA very rapidly and with high processivity.
Highlights:
- Encodes an Asp-1080-to-Ala mutation in the RecB subunit - Abolishes the nuclease activity, but retains rapid and processive helicase activity.
RecBCD (EC 3.1.11.5, Exonuclease V, Escherichia coli exonuclease V, E. coli exonuclease V, gene recBC endoenzyme, RecBC deoxyribonuclease, gene recBC DNase, gene recBCD enzymes) is an enzyme of the E. coli bacterium that initiates recombinational repair from potentially lethal double strand breaks in DNA which may result from ionizing radiation, replication errors, endonucleases, oxidative damage, and a host of other factors. The RecBCD enzyme is both a helicase that unwinds, or separates the strands of DNA, and a nuclease that makes single-stranded nicks in DNA.
From the laboratory of Douglas A. Julin, PhD, University of Maryland, College Park.
| Product Type: | Plasmid |
| Name: | thyA-recC-ptrIII-recB-recD-argA (E. coli) |
| Accession ID: | P08394, P04993, P07648 |
| Organism: | E. coli |
| Antibiotic Resistance: | Chloramphenicol |
| Fusion Tag(s): | None |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Chloramphenicol |
| Cloning Site 5': | BamHI |
| Cloning Site 3': | BamHI |
| 5' Sequencing Primer: | 18,344 bp |
| 3' Sequencing Primer: | pACYC184, 4,258 bp |
| Insert Size: | Low |
| Shipped: | Ambient temperature |
- Yu M, Souaya J, Julin DA. Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme. J Mol Biol. 1998 Nov 6;283(4):797-808. PubMed PMID: 9790841.
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