The pLU-JARID1B promoter-EGFP lentiviral plasmid drives cytoplasmic EGFP expression controlled by the co-cloned human JARID1B promoter, allowing for the isolation of live JARID1B-positive cells.
JARID1B (KDM5B/PLU-1/RBP2-H1) is a member of the highly conserved family of jumonji/ARID1 (JARID1) histone 3 K4 (H3K4) demethylases which are involved in tissue development, cancer, and normal stem cell biology. In normal adult tissues, JARID1B is marginally expressed with dramatic peak expression levels in regenerative tissues like testis and bone marrow. In cancer, JARID1B functions as a transcriptional regulator of oncogenes via direct interaction with promoter sites. Depending on the cancer context, JARID1B is associated with either positive (melanoma) or negative (breast cancer) cell cycle control.
From the laboratory of Meenhard Herlyn, DVM, DSc, The Wistar Institute.
Part of The Investigator's Annexe program.
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|Gene/insert name:||JARID1B promoter|
|Fusion Tag(s):||EGFP (C terminal on backbone)|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||Unknown|
|Cloning Site 3':||EGFP-N|
|Insert Size:||249 bp|
|High or low copy:||High|
|Shipped:||Ambient temperature as Liquid|
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