The pGEM-Gpr177 is a plasmid vector encoding the mammalian homolog of Drosophilia Wntless. This probe can be used to generate RNA sense and anti-sense probes to recognize Wntless in various tissues by in situ hybridization.
The Wnt signaling pathways are a group of signal transduction pathways made of proteins that pass signals from outside of a cell through cell surface receptors to the inside of the cell. G protein-coupled receptor 177 (Gpr177) is the mammalian homolog of Drosophila Wntless (Wls; aka Evi, Srt) and is required for Wnt secretion in Wnt-producing cells. Wntless binds to lipid modified Wnts and is mainly localized in the Golgi and trafficking vesicles to modulates intracellular sorting of Wnt proteins. It has been demonstrated that genetic inactivation of Wntless in mice causes similar defects to the loss of canonical Wnt as well as non-canonical Wnt signaling. However, the loss of Wntless does not affect Wnt expression but Wnt secretion in the signal-producing cells. Wntless is also associated with the retromer complex to recycle Wnt from the plasma membrane and the expression pattern of Wntless provides critical information for Wnt-producing cells and source of Wnt in development and disease.
From the laboratory of Wei Hsu, PhD, University of Rochester.
Part of The Investigator's Annexe program.
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||Spel|
|Cloning Site 3':||EcoRI|
|Insert Size:||603 bp|
|Vector Backbone and Size:||pGEM, 3015|
|High or low copy:||High|
|Shipped:||Ambient temperature as liquid|
Use SP6 and T7 polymerases to produce sense and anti-sense RNA probes, respectively.
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