This rabbit polyclonal antibody was generated against a synthetic peptide and and is reactive for the sequence CDGPTEIYKLTRKEAQE of human, mouse and rat G protein-coupled receptor 177 (Wntless/Gpr177).
G protein-coupled receptor 177 (Gpr177), is the mammalian homologue of Drosophila Wntless (Wls; aka Evi, Srt) is required for Wnt secretion in Wnt-producing cells [1-3]. Wntless binds to lipid modified Wnts, and is mainly localized in the Golgi and trafficking vesicles to modulates intracellular sorting of Wnt proteins . It has been demonstrated that genetic inactivation of Wntless in mice causes similar defects to the loss of canonical Wnt [4-10] as well as non-canonical Wnt signaling . However, the loss of Wntless does not affect Wnt expression but Wnt secretion in the signal-producing cells [1, 6]. Wntless is also associated with the retromer complex to recycle Wnt from the plasma membrane [11-15]. The expression pattern of Wntless provides critical information for Wnt-producing cells and source of Wnt in development and disease.
From the laboratory of Wei Hsu, PhD, University of Rochester.
Part of The Investigator's Annexe program.
|Molecular Weight:||~60 kDa|
|Reactivity:||Human, mouse and rat (others not tested)|
|Purification Method:||Affinity purified with peptide column|
|Method Used to Determine Concentration:||OD 280|
|Tested Applications:||WB: (1:1,000), IF: (1:1,000), IHC-parrafin (1:3,000)|
Co-immunostaining of Wntless (green) and K14 (red) counterstained with DAPI (blue) in paraffin-embedded sections of control (A) and mutant with deletion of Gpr177/Wntless in the epidermis but not the dermis (B) using Wntless Rabbit polyclonal.Adapted from: Fu J. et al., J Invest Dermatol. 2013 Apr;133(4):890-8.
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