This IgG2a monoclonal antibody [CAb 1] was raised against GST-fusion protein and reacts with human and mouse inhibitor of growth protein 1 or p33ING1b.
The ING genes encode a family of at least seven proteins with conserved plant homeodomain (PHD)-type zinc fingers in their C-termini. The founding member, ING1, is capable of binding to and affecting the activity of histone acetyltransferase (HAT), histone deacetylase (HDAC), and factor acetyltransferase (FAT) protein complexes. Upon UV irradiation, ING1 and ING2 bind stress induced signaling phospholipids such as phosphatidylinositol 5-monophosphate and cause cell cycle arrest. ING1b interacts with proliferating cell nuclear antigen to promote DNA repair or induce apoptosis in cells to prevent tumorigenesis, depending upon the severity of DNA damage. ING proteins, which are down-regulated in a broad variety of cancer types, are able to restrict cell growth and proliferation, induce apoptosis, and modulate cell cycle progression, which strongly supports the notion that ING family proteins act as type II tumor suppressors. The ING proteins regulate chromatin structure through specific binding to histones H3 and H4 via their plant homeodomains (PHDs) and recruitment of HAT & HDAC complexes to particular regions of chromatin, thus playing central roles in reading of the "histone code".
From the laboratory of Karl Riabowol, PhD, University of Calgary.
Part of The Investigator's Annexe program.
|Accession ID:||Q9UK53, AAF07921|
|Molecular Weight:||37 kDa|
|Clone Name:||CAb 1|
|Reactivity:||Human and Mouse|
|Purification Method:||Anti-bovine IgG column; Protein G column|
|Method Used to Determine Concentration:||OD 280 nm|
|Buffer:||PBS, pH 7.4, 0.01% thimerosal|
|Tested Applications:||ELISA, IF, WB|
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