NIH 3T3 Duo-Parental Cell Line

The NIH 3T3 Duo-Parental Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.

NIH3T3 cells which contain integrated into chromosome 7 the ISceI restriction endonuclease site adjacent to an array of the Lac-operator DNA sequence (LacO, 256 copies) and three integrations on chromosomes 1 and 10 (integrations on two chromosome homologs) of the TetO-ISceI-TetO array (TetO, 96 copies). This cell line does not coexpress the fluorescent repressors LacR-GFP and TetR-mCherry.

From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
NIH 3T3 Duo-Parental Cell Line
1 vial 4-6 weeks
Regular Price:$910.00
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Product Type: Cell Line
Name: NIH 3T3 Duo-Parental
Cell Type: NIH3T3 mouse embryonic fibroblasts
Accession ID: CVCL_HC50
Organism: Mouse
Source: mouse embryo (NIH3T3 cells)
Morphology: Fibroblast
Biosafety Level: BSL1
Subculturing: Split 1 to 3 when ?90% confluency
Growth Conditions: High-glucose DMEM, 15% FBS, P/S, 2mM Glutamine
Cryopreservation: 15% DMSO in FBS
Storage: Liquid nitrogen
Shipped: Dry ice

From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.

We routinely culture the cells in the absence of the drugs for selection without a major loss of cells containing the arrays.

  1. Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T. Spatial dynamics of chromosome translocations in living cells. Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
  2. Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014 Oct;9(10):2476-92. doi: 10.1038/nprot.2014.167. Epub 2014 Sep 25.

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