This rabbit polyclonal antibody  was generated against a synthetic peptide corresponding to residues 231-245 (SAEEKKEQTIEIKEE) of human CD20 fused to ovalbumin, and is specific for human CD20 (intracellular C-terminal region).
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
Part of The Investigator's Annexe program.
|Molecular Weight:||33-35 kDa|
|Immunogen:||Peptide conjugated to ovalbumin|
|Epitope:||C-terminal residues 231-245; SAEEKKEQTIEIKEE|
|Tested Applications:||WB (1:2,000)|
Western Blot of CD20 N and C-terminus
Western blots of human Ramos B-cells lysates probed with rabbit anti-CD20N (targeting the N-terminal residues 25-41) and rabbit anti-CD20C (targeting the C-terminal residues 231-245), pre-incubated with immunizing peptide (lanes 2 and 5), irrelevant peptide (lanes 3 and 6) or no peptide (lanes 1 and 4).
Adapted from: Polyak MJ, et al. J Immunol. 1998 Oct 1;161(7):3242-8.
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