Anti-CD20, C-Terminal [1440] Antibody

This rabbit polyclonal antibody [1440] was generated against a synthetic peptide corresponding to residues 231-245 (SAEEKKEQTIEIKEE) of human CD20 fused to ovalbumin, and is specific for human CD20 (intracellular C-terminal region).


  • Reacts with intracellular residues 231-245 of human CD20
  • Valuable for both pre-clinical and clinical research applications
  • Suitable for Western Blot and Immunoprecipitation applications

CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.

From the laboratory of Julie P. Deans, PhD, University of Calgary.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
Anti-CD20, C-Terminal [1440] Antibody
100uL In stock
Regular Price:$299.00

Product Type: Antibody
Antigen: CD20 (CD20-C)
Molecular Weight: 33-35 kDa
Clonality: Polyclonal
Clone Name: 1440
Reactivity: Human
Immunogen: Peptide conjugated to ovalbumin
Species Immunized: Rabbit
Epitope: C-terminal residues 231-245; SAEEKKEQTIEIKEE
Buffer: Serum
Tested Applications: WB (1:2,000)
Storage: -80C
Shipped: Dry ice


Western Blot of CD20 N and C-terminus

Western blots of human Ramos B-cells lysates probed with rabbit anti-CD20N (targeting the N-terminal residues 25-41) and rabbit anti-CD20C (targeting the C-terminal residues 231-245), pre-incubated with immunizing peptide (lanes 2 and 5), irrelevant peptide (lanes 3 and 6) or no peptide (lanes 1 and 4).

Adapted from: Polyak MJ, et al. J Immunol. 1998 Oct 1;161(7):3242-8.

From the laboratory of Julie P. Deans, PhD, University of Calgary.
  1. Polyak MJ, Tailor SH, Deans JP. Identification of a cytoplasmic region of CD20 required for its redistribution to a detergent-insoluble membrane compartment. J Immunol. 1998 Oct 1;161(7):3242-8.
  2. Deans JP, Robbins SM, Polyak MJ, Savage JA. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8.
  3. Popoff IJ, Savage JA, Blake J, Johnson P, Deans JP. The association between CD20 and Src-family Tyrosine kinases requires an additional factor. Mol Immunol. 1998 Mar;35(4):207-14.
  4. Polyak MJ, Deans JP. Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62.
  5. Polyak MJ, Li H, Shariat N, Deans JP. CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins. J Biol Chem. 2008 Jul 4;283(27):18545-52.

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