Canine Melanoma Cell Line (TLM-1)

TLM-1 is a canine melanoma cell line derived from an oral tumor of a 12-year old male Gordon setter.


  • Grows in vitro and forms heterotopic tumor xenografts in immunodeficient mice
  • Extensively characterized and published on

Malignant melanoma is a type of cancer in dogs that affects pigmented-producing cells known as melanocytes. Most malignant melanomas are diagnosed in the skin and mucocutaneous junctions. They tend to grow extremely fast and are likely to spread to other organs, including the lungs and liver.

From the laboratory of Jaime F. Modiano, VMD, PhD, University Minnesota, Twin Cities.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Canine Melanoma Cell Line (TLM-1)
1 vial In stock
Regular Price:$640.00
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Product Type: Cell Line
Name: TLM-1 (also called TLM1)
Cell Type: Melanoma
Accession ID: CVCL_0C83
Organism: Dog
Source: Oral tumor (malignant melanoma)
Morphology: Epithelioid to spindle
Biosafety Level: 2
Growth Conditions: DMEM, 10mM HEPES, 10% FBS, 100ug/mL Primocin
Cryopreservation: 90%FBS/10% DMSO
Mycoplasma Tested: Yes
Storage: Liquid nitrogen
Shipped: Dry ice

From the laboratory of Jaime F. Modiano, VMD, PhD, University Minnesota, Twin Cities.
  1. Ritt MG, Wojcieszyn J, Modiano JF. Functional loss of p21/Waf-1 in a case of benign canine multicentric melanoma. Vet Pathol. 1998 Mar;35(2):94-101.
  2. Modiano JF, Ritt MG, Wojcieszyn J, Smith R 3rd. Growth arrest of melanoma cells is differentially regulated by contact inhibition and serum deprivation. DNA Cell Biol. 1999 May;18(5):357-67.
  3. Ritt MG, Mayor J, Wojcieszyn J, Smith R, Barton CL, Modiano JF. Sustained nuclear localization of p21/WAF-1 upon growth arrest induced by contact inhibition. Cancer Lett. 2000 Sep 29;158(1):73-84.
  4. Koenig A, Wojcieszyn J, Weeks BR, Modiano JF. Expression of S100a, vimentin, NSE, and melan A/MART-1 in seven canine melanoma cells lines and twenty-nine retrospective cases of canine melanoma. Vet Pathol. 2001 Jul;38(4):427-35.
  5. Koenig A, Bianco SR, Fosmire S, Wojcieszyn J, Modiano JF. Expression and significance of p53, rb, p21/waf-1, p16/ink-4a, and PTEN tumor suppressors in canine melanoma. Vet Pathol. 2002 Jul;39(4):458-72.
  6. Bianco SR, Sun J, Fosmire SP, Hance K, Padilla ML, Ritt MG, Getzy DM, Duke RC, Withrow SJ, Lana S, Matthiesen DT, Dow SW, Bellgrau D, Cutter GR, Helfand SC, Modiano JF. Enhancing antimelanoma immune responses through apoptosis. Cancer Gene Ther. 2003 Sep;10(9):726-36.
  7. Lin W, Modiano JF, Ito D. Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells. J Vet Sci. 2017 Mar 30;18(1):101-104. doi: 10.4142/jvs.2017.18.1.101. PubMed PMID: 27456773; PubMed Central PMCID: PMC5366293.

If you publish research with this product, please let us know so we can cite your paper.