Anti-MuLV Transmembrane Protein [42/114] Antibody (supernatant)

This mouse IgM monoclonal antibody [42/114]  was generated against AKR K36 and AKR SL3 leukemia and is specific for rat murine leukemia virus (MuLV) transmembrane protein.

Highlights:

  • Reacts with rat murine leukemia virus transmembrane protein
  • Suitable for Western Blot and Immunoprecipitation applications

Murine leukemia viruses also known as MuLVs are retroviruses named for their ability to cause cancer in mouse hosts. They have multiple applications including gene therapy, cancer studies, as a model for retroviruses in viral clearance studies and also can used in biotechnology for reverse transcriptase.

From the laboratory of Abraham Pinter, PhD, Rutgers University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ED8001
Anti-MuLV Transmembrane Protein [42/114] Antibody (supernatant)
5mL  In stock
Regular Price:$376.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Murine leukemia virus transmembrane protein
Isotype: IgM
Clonality: Monoclonal
Clone Name: 42/114
Reactivity: Rat
Immunogen: AKR K36 and AKR SL3 leukemia
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IP
Storage: -80C
Shipped: Dry Ice

Documentation

Notes

Original characterization of antibodies was done by ammonium sulphate precipitations followed by radioimmunoprecipitation assays.

Data

Immunoprecipitation

Precipitation of intact virions with 44-114 antibody. Supernatants from NIH 3T3 cells infected with MuLV were immunoprecipitated in presence (+) and absence (-) of of NP-40 with 20u/mL of antibody. Precipitates were analyzed by SDS-Page and autoradiography. TM refers to the transmembrane subunit of Env. p15E and CA refers to the MuLV capsid protein, p30.

Adapted from: Virol. 2003 Apr;77(7):3993-4003.

Provider
From the laboratory of Abraham Pinter, PhD, Rutgers University.
References
  1. Lostrom, ME , Stone, MR, Tam M, Burnette WN, Pinter A, Nowinski RC. Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the p15(E) and gp70 envelope proteins. Virology 1979 Oct 30;98(2):336-50.
  2. Hammerling U., Pinter A, and O'donnell PV, (1980) Monoclonal Antibodies as a tool in research on oncogenic viruses. p. 255-256. In G. A. Gerth (ed). The role of viruses in human cancer (Elsevier, Amsterdam).
  3. Pinter, A., Honnen, WJ, Tung, JS, O'Donnell, PV. Structural domains of endogenous murine leukemia virus gp70s containing specific antigenic determinants defined by monoclonal antibodies. Virology 1982 116(2):s 499-516
  4. Burkhart MD, Kayman SC, He Y, Pinter A. Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. J Virol. 2003 Apr;77(7):3993-4003.
  5. Timilsina U, Umthong S, Lynch B, Stablewski A, Stavrou S. SERINC5 Potently Restricts Retrovirus Infection In Vivo. mBio. 2020;11(4):e00588-20. Published 2020 Jul 14. View article
  6. Umthong S, Lynch B, Timilsina U, Waxman B, Ivey EB, Stavrou S. Elucidating the Antiviral Mechanism of Different MARCH Factors. mBio. 2021 Mar 2;12(2):e03264-20. View article 

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